glinski_metabolites
tested hypotheses
- observed vivo versus in vitro degradation rates are the same, or observed vivo rates are lower than in vitro rates (due to other degradation processes, uptake kinetics?)
- first order kinetics are michaelis-menton, second order
- are in vitro indicative of in vivo
things to do
- michaelis menton first order comparison for both experiments
- non-linear goodness of fit
install and load supporting libraries
## nodename
## "DZ2626UTPURUCKE"## [1] "R version 3.2.3 (2015-12-10)"##
## Attaching package: 'MASS'
##
## The following object is masked from 'package:dplyr':
##
## select
##
##
## 'drc' has been loaded.
##
## Please cite R and 'drc' if used for a publication,
## for references type 'citation()' and 'citation('drc')'.## [1] "list of loaded packages: "## [1] "scatterplot3d" "ggplot2" "drc" "MASS"
## [5] "xtable" "zoo" "knitr" "dplyr"
## [9] "rmarkdown" "stats" "graphics" "grDevices"
## [13] "utils" "datasets" "methods" "base"import experimental exposure and microsomal data
Load csv files with experimental data and microsome data sets. The beolw may return false but still be OK if rstudio does not have privileges to data directory (e.g., attached drive).
## [1] "Root directory location: k:/git/glinski_metabolites/"## [1] "check to see if R can access files OK: FALSE"## [1] "outliers:"## time parent analyte matrix conc replicate microMexp
## 96 30 atrazine atrazine microsomes 158.6508 1 125
## 99 60 atrazine atrazine microsomes 170.6522 1 125
## 125 0 atrazine atrazine microsomes 152.8226 1 200
## 126 0 atrazine atrazine microsomes 135.8870 2 200
## 127 0 atrazine atrazine microsomes 146.1521 3 200
## 128 15 atrazine atrazine microsomes 200.8988 1 200
## 129 15 atrazine atrazine microsomes 134.8156 2 200
## 130 15 atrazine atrazine microsomes 211.5786 3 200
## 131 30 atrazine atrazine microsomes 193.1223 1 200
## 132 30 atrazine atrazine microsomes 210.4726 2 200
## 133 30 atrazine atrazine microsomes 213.5141 3 200
## 134 60 atrazine atrazine microsomes 197.4426 1 200
## 135 60 atrazine atrazine microsomes 146.8087 2 200
## 136 60 atrazine atrazine microsomes 201.9011 3 200
## 137 90 atrazine atrazine microsomes 127.9031 1 200
## 138 90 atrazine atrazine microsomes 203.6292 2 200
## 139 90 atrazine atrazine microsomes 128.0068 3 200experimental exposure data structure
Check out structure of imported data sets.
str(micro)## 'data.frame': 352 obs. of 6 variables:
## $ time : int 2 2 2 2 4 4 4 4 12 12 ...
## $ parent : Factor w/ 3 levels "atrazine","fipronil",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ analyte : Factor w/ 8 levels "atrazine","dea",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ matrix : Factor w/ 2 levels "amphib","soil": 1 1 1 1 1 1 1 1 1 1 ...
## $ conc : num 3.07 3.69 4.7 1.89 7.62 ...
## $ replicate: int 1 2 3 4 1 2 3 4 1 2 ...Set time and replicate fields as factors for later statistical inference.
microsome experiment data structure
Check out structure of imported data set for the microsome analysis.
str(microsome)## 'data.frame': 1106 obs. of 7 variables:
## $ time : int 0 0 0 15 15 15 30 30 30 60 ...
## $ parent : Factor w/ 3 levels "atrazine","fipronil",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ analyte : Factor w/ 8 levels "atrazine","dea",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ matrix : Factor w/ 1 level "microsomes": 1 1 1 1 1 1 1 1 1 1 ...
## $ conc : num 1.208 1.596 1.362 0.551 1.347 ...
## $ replicate: int 1 2 3 1 2 3 1 2 3 1 ...
## $ microMexp: num 0.712 0.712 0.712 0.712 0.712 0.712 0.712 0.712 0.712 0.712 ...Set time and replicate fields as factors for later statistical inference.
## 'data.frame': 1106 obs. of 7 variables:
## $ time : int 0 0 0 15 15 15 30 30 30 60 ...
## $ parent : Factor w/ 3 levels "atrazine","fipronil",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ analyte : Factor w/ 8 levels "atrazine","dea",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ matrix : Factor w/ 1 level "microsomes": 1 1 1 1 1 1 1 1 1 1 ...
## $ conc : num 1.208 1.596 1.362 0.551 1.347 ...
## $ replicate: Factor w/ 4 levels "1","2","3","4": 1 2 3 1 2 3 1 2 3 1 ...
## $ microMexp: num 0.712 0.712 0.712 0.712 0.712 0.712 0.712 0.712 0.712 0.712 ...analyze microsomal data
The microsomal experiment has 3-4 replicates for each pesticide. The experiment progressively increases the concentration of the substrate (microMexp) and measures the enzyme velocity. At each substrate concentration the reaction is quenched after a specific period of time (0, 15, 30, 60, 90 mins). This time series data is used to estimate a slope associated with each substrate concentration. Substrate concentration and linear velocity slopes are then fit using Michaelis-Menten kinetics to estimate reaction rates for each pesticide.
atrazine
The above figure shows a three-dimensional plot of pesticide substrate concentration, observed concentration and experiment time. We need to condition the pesticide data on the substrate concentrations used in the experiment and regress the observed concentrations over the 90 min period in order to estimate the linear slopes for the Michaelis-Menten kinetic rate constants.
## [1] "Substrates:"## [1] "##################################################"
## [1] "Substrate = 0.712"
## [1] "Indices = 1" "Indices = 2" "Indices = 3" "Indices = 4"
## [5] "Indices = 5" "Indices = 6" "Indices = 7" "Indices = 8"
## [9] "Indices = 9" "Indices = 10" "Indices = 11" "Indices = 12"
## [13] "Indices = 13" "Indices = 14" "Indices = 15"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 1.208049891" "Concs = 1.59580743" "Concs = 1.362262655"
## [4] "Concs = 0.551435364" "Concs = 1.346911275" "Concs = 1.212444791"
## [7] "Concs = 1.13491833" "Concs = 1.082466929" "Concs = 1.113889522"
## [10] "Concs = 0.918015161" "Concs = 0.819916078" "Concs = 0.830401682"
## [13] "Concs = 0.60193292" "Concs = 0.514615471" "Concs = 0.543046503"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 1.309205 -0.008208
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.63464 -0.01209 0.02637 0.06251 0.28660
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 1.309205 0.083093 15.756 7.52e-10 ***
## Time[ind] -0.008208 0.001641 -5.003 0.000242 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.2053 on 13 degrees of freedom
## Multiple R-squared: 0.6582, Adjusted R-squared: 0.6319
## F-statistic: 25.03 on 1 and 13 DF, p-value: 0.0002416
## (Intercept) Time[ind]
## 1.309205033 -0.008208481
## 2.5 % 97.5 %
## (Intercept) 1.12969277 1.488717294
## Time[ind] -0.01175294 -0.004664018
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 10"
## [1] "Indices = 16" "Indices = 17" "Indices = 18" "Indices = 19"
## [5] "Indices = 20" "Indices = 21" "Indices = 22" "Indices = 23"
## [9] "Indices = 24" "Indices = 25" "Indices = 26" "Indices = 27"
## [13] "Indices = 28" "Indices = 29" "Indices = 30"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 60" "Times = 90" "Times = 90"
## [1] "Concs = 8.602230146" "Concs = 8.531210299" "Concs = 8.218650517"
## [4] "Concs = 8.453642708" "Concs = 7.964608184" "Concs = 7.94451139"
## [7] "Concs = 7.680602046" "Concs = 7.527336918" "Concs = 7.478498249"
## [10] "Concs = 5.659397741" "Concs = 6.36413415" "Concs = 6.660240451"
## [13] "Concs = 6.34249619" "Concs = 5.477249479" "Concs = 5.670032215"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 8.5223 -0.0347
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.78076 -0.06665 0.00889 0.13964 0.45186
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 8.522319 0.119538 71.29 < 2e-16 ***
## Time[ind] -0.034703 0.002512 -13.82 3.79e-09 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.2912 on 13 degrees of freedom
## Multiple R-squared: 0.9362, Adjusted R-squared: 0.9313
## F-statistic: 190.9 on 1 and 13 DF, p-value: 3.789e-09
## (Intercept) Time[ind]
## 8.52231931 -0.03470261
## 2.5 % 97.5 %
## (Intercept) 8.26407296 8.78056567
## Time[ind] -0.04012886 -0.02927636
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 3.1684"
## [1] "Indices = 31" "Indices = 32" "Indices = 33" "Indices = 34"
## [5] "Indices = 35" "Indices = 36" "Indices = 37" "Indices = 38"
## [9] "Indices = 39" "Indices = 40" "Indices = 41" "Indices = 42"
## [13] "Indices = 43" "Indices = 44" "Indices = 45"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 3.365067675" "Concs = 3.642810527" "Concs = 3.434256239"
## [4] "Concs = 3.840288882" "Concs = 3.471244145" "Concs = 4.21892194"
## [7] "Concs = 3.04149649" "Concs = 3.107056916" "Concs = 2.539792287"
## [10] "Concs = 2.350740689" "Concs = 2.184285874" "Concs = 1.868856626"
## [13] "Concs = 1.487945081" "Concs = 1.746448606" "Concs = 1.279189407"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 3.76182 -0.02538
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.46055 -0.26287 0.01058 0.10930 0.83784
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 3.761823 0.145888 25.786 1.51e-12 ***
## Time[ind] -0.025383 0.002881 -8.812 7.64e-07 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.3605 on 13 degrees of freedom
## Multiple R-squared: 0.8566, Adjusted R-squared: 0.8456
## F-statistic: 77.65 on 1 and 13 DF, p-value: 7.644e-07
## (Intercept) Time[ind]
## 3.76182347 -0.02538282
## 2.5 % 97.5 %
## (Intercept) 3.44665102 4.07699592
## Time[ind] -0.03160589 -0.01915975
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 50"
## [1] "Indices = 46" "Indices = 47" "Indices = 48" "Indices = 49"
## [5] "Indices = 50" "Indices = 51" "Indices = 52" "Indices = 53"
## [9] "Indices = 54" "Indices = 55" "Indices = 56" "Indices = 57"
## [13] "Indices = 58" "Indices = 59" "Indices = 60"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 32.61853939" "Concs = 28.61877014" "Concs = 24.9941227"
## [4] "Concs = 30.1352595" "Concs = 28.31360828" "Concs = 26.0438183"
## [7] "Concs = 30.34766105" "Concs = 27.74701672" "Concs = 25.6412683"
## [10] "Concs = 28.51624079" "Concs = 26.35221344" "Concs = 24.78295594"
## [13] "Concs = 26.56519331" "Concs = 24.44743091" "Concs = 24.01565934"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 28.88318 -0.04121
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -3.8891 -1.3931 -0.0584 1.6306 3.7354
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 28.88318 0.86801 33.275 5.74e-14 ***
## Time[ind] -0.04121 0.01714 -2.404 0.0318 *
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 2.145 on 13 degrees of freedom
## Multiple R-squared: 0.3078, Adjusted R-squared: 0.2546
## F-statistic: 5.782 on 1 and 13 DF, p-value: 0.03182
## (Intercept) Time[ind]
## 28.8831817 -0.0412102
## 2.5 % 97.5 %
## (Intercept) 27.00795252 30.758410981
## Time[ind] -0.07823653 -0.004183871
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 75"
## [1] "Indices = 61" "Indices = 62" "Indices = 63" "Indices = 64"
## [5] "Indices = 65" "Indices = 66" "Indices = 67" "Indices = 68"
## [9] "Indices = 69" "Indices = 70" "Indices = 71" "Indices = 72"
## [13] "Indices = 73" "Indices = 74" "Indices = 75"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 31.69355406" "Concs = 32.50417088" "Concs = 39.73678741"
## [4] "Concs = 29.59548976" "Concs = 36.16487256" "Concs = 37.40302159"
## [7] "Concs = 27.6465584" "Concs = 28.94210726" "Concs = 34.71955504"
## [10] "Concs = 31.19094038" "Concs = 35.96712185" "Concs = 33.12001232"
## [13] "Concs = 31.51869676" "Concs = 32.99095623" "Concs = 22.85692843"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 34.36984 -0.05042
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -6.975 -3.296 1.687 2.855 5.367
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 34.36984 1.62052 21.209 1.81e-11 ***
## Time[ind] -0.05042 0.03200 -1.576 0.139
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 4.004 on 13 degrees of freedom
## Multiple R-squared: 0.1604, Adjusted R-squared: 0.0958
## F-statistic: 2.483 on 1 and 13 DF, p-value: 0.1391
## (Intercept) Time[ind]
## 34.36983650 -0.05042184
## 2.5 % 97.5 %
## (Intercept) 30.8689187 37.87075426
## Time[ind] -0.1195473 0.01870365
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 100"
## [1] "Indices = 76" "Indices = 77" "Indices = 78" "Indices = 79"
## [5] "Indices = 80" "Indices = 81" "Indices = 82" "Indices = 83"
## [9] "Indices = 84" "Indices = 85" "Indices = 86" "Indices = 87"
## [13] "Indices = 88" "Indices = 89" "Indices = 90"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 76.46874561" "Concs = 67.44424419" "Concs = 60.20571288"
## [4] "Concs = 73.12305661" "Concs = 62.97208244" "Concs = 58.17491784"
## [7] "Concs = 70.9357471" "Concs = 60.89975615" "Concs = 54.39425099"
## [10] "Concs = 67.30936536" "Concs = 56.7524779" "Concs = 50.73568923"
## [13] "Concs = 64.11432499" "Concs = 53.06960934" "Concs = 51.00465026"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 66.948 -0.131
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -8.625 -5.449 -2.092 8.028 9.521
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 66.94770 2.83785 23.591 4.69e-12 ***
## Time[ind] -0.13096 0.05603 -2.337 0.0361 *
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 7.012 on 13 degrees of freedom
## Multiple R-squared: 0.2959, Adjusted R-squared: 0.2417
## F-statistic: 5.462 on 1 and 13 DF, p-value: 0.03608
## (Intercept) Time[ind]
## 66.9476963 -0.1309587
## 2.5 % 97.5 %
## (Intercept) 60.816898 73.078494940
## Time[ind] -0.252011 -0.009906267
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 125"
## [1] "Indices = 91" "Indices = 92" "Indices = 93" "Indices = 94"
## [5] "Indices = 95" "Indices = 96" "Indices = 97" "Indices = 98"
## [9] "Indices = 99" "Indices = 100" "Indices = 101" "Indices = 102"
## [1] "Times = 0" "Times = 0" "Times = 15" "Times = 15" "Times = 15"
## [6] "Times = 30" "Times = 30" "Times = 60" "Times = 60" "Times = 90"
## [11] "Times = 90" "Times = 90"
## [1] "Concs = 88.09234635" "Concs = 85.70476499" "Concs = 107.9737675"
## [4] "Concs = 85.24148342" "Concs = 85.54901737" "Concs = 83.6386001"
## [7] "Concs = 83.07467838" "Concs = 78.21161933" "Concs = 84.54185693"
## [10] "Concs = 80.88478508" "Concs = 79.00424811" "Concs = 78.20108166"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 90.1940 -0.1257
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -4.4892 -3.1378 -2.4307 0.5629 19.6649
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 90.1940 3.1499 28.634 6.28e-11 ***
## Time[ind] -0.1257 0.0592 -2.123 0.0597 .
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 6.892 on 10 degrees of freedom
## Multiple R-squared: 0.3107, Adjusted R-squared: 0.2418
## F-statistic: 4.507 on 1 and 10 DF, p-value: 0.05972
## (Intercept) Time[ind]
## 90.1939928 -0.1256761
## 2.5 % 97.5 %
## (Intercept) 83.1756334 97.212352214
## Time[ind] -0.2575766 0.006224427
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 150"
## [1] "Indices = 103" "Indices = 104" "Indices = 105" "Indices = 106"
## [5] "Indices = 107" "Indices = 108" "Indices = 109" "Indices = 110"
## [9] "Indices = 111" "Indices = 112" "Indices = 113" "Indices = 114"
## [13] "Indices = 115" "Indices = 116" "Indices = 117" "Indices = 118"
## [17] "Indices = 119" "Indices = 120" "Indices = 121" "Indices = 122"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 0" "Times = 15"
## [6] "Times = 15" "Times = 15" "Times = 15" "Times = 30" "Times = 30"
## [11] "Times = 30" "Times = 30" "Times = 60" "Times = 60" "Times = 60"
## [16] "Times = 60" "Times = 90" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 111.7791798" "Concs = 108.954701" "Concs = 113.7158271"
## [4] "Concs = 107.5512697" "Concs = 110.8050341" "Concs = 114.7205562"
## [7] "Concs = 107.1291095" "Concs = 110.2111065" "Concs = 106.4113993"
## [10] "Concs = 109.2588435" "Concs = 108.8110859" "Concs = 98.45121644"
## [13] "Concs = 105.7397626" "Concs = 103.5578664" "Concs = 109.569591"
## [16] "Concs = 103.2077499" "Concs = 96.26533592" "Concs = 104.9741825"
## [19] "Concs = 105.7942757" "Concs = 95.31462118"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 110.8882 -0.1097
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -9.1469 -1.9786 0.9295 1.9524 5.4774
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 110.88819 1.37221 80.810 < 2e-16 ***
## Time[ind] -0.10967 0.02709 -4.048 0.000755 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 3.915 on 18 degrees of freedom
## Multiple R-squared: 0.4765, Adjusted R-squared: 0.4474
## F-statistic: 16.38 on 1 and 18 DF, p-value: 0.0007553
## (Intercept) Time[ind]
## 110.888186 -0.109668
## 2.5 % 97.5 %
## (Intercept) 108.0052898 113.77108184
## Time[ind] -0.1665906 -0.05274528
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 250"
## [1] "Indices = 123" "Indices = 124" "Indices = 125" "Indices = 126"
## [5] "Indices = 127" "Indices = 128" "Indices = 129" "Indices = 130"
## [9] "Indices = 131" "Indices = 132" "Indices = 133" "Indices = 134"
## [13] "Indices = 135" "Indices = 136" "Indices = 137"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 31" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 135.98518" "Concs = 118.5627805" "Concs = 138.8461629"
## [4] "Concs = 111.0650322" "Concs = 110.394181" "Concs = 107.7699691"
## [7] "Concs = 110.0390245" "Concs = 133.1636589" "Concs = 106.6453068"
## [10] "Concs = 125.4094086" "Concs = 132.3152295" "Concs = 129.5529012"
## [13] "Concs = 107.9475473" "Concs = 109.2892497" "Concs = 110.9269157"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 122.96364 -0.09649
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -13.746 -10.241 -4.401 12.700 15.883
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 122.96364 4.82709 25.474 1.76e-12 ***
## Time[ind] -0.09649 0.09524 -1.013 0.329
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 11.91 on 13 degrees of freedom
## Multiple R-squared: 0.07318, Adjusted R-squared: 0.001888
## F-statistic: 1.026 on 1 and 13 DF, p-value: 0.3295
## (Intercept) Time[ind]
## 122.96363586 -0.09648804
## 2.5 % 97.5 %
## (Intercept) 112.5353325 133.3919392
## Time[ind] -0.3022315 0.1092554
## [1] "##################################################"## 'data.frame': 9 obs. of 2 variables:
## $ sub: num 0.712 10 3.168 50 75 ...
## $ vel: num 41 174 127 206 252 ...## sub vel
## 1 0.7120 41.04241
## 2 10.0000 173.51305
## 3 3.1684 126.91411
## 4 50.0000 206.05101
## 5 75.0000 252.10918
## 6 100.0000 654.79328
## 7 125.0000 628.38045
## 8 150.0000 548.33976
## 9 250.0000 482.44019
We are interested in the reaction rate, v, which is a function of available substrate (pesticide) and the presence of an enzyme. We get Vmax from the asymptote and K = 0.5*Vmax. Biochemical reactions with a single substrate are typically assumed to follow Michaelis-Menten kinetics even if they deviate from the basic model assumptions.
We are fitting the 2-parameter model.
Used to estimate slopes.
dea
The above figure shows a three-dimensional plot of pesticide substrate concentration, observed concentration and experiment time. We need to condition the pesticide data on the substrate concentrations used in the experiment and regress the observed concentrations over the 90 min period in order to estimate the linear slopes for the Michaelis-Menten kinetic rate constants.
## [1] "Substrates:"## [1] "##################################################"
## [1] "Substrate = 50"
## [1] "Indices = 1" "Indices = 2" "Indices = 3" "Indices = 4"
## [5] "Indices = 5" "Indices = 6" "Indices = 7" "Indices = 8"
## [9] "Indices = 9" "Indices = 10" "Indices = 11" "Indices = 12"
## [13] "Indices = 13" "Indices = 14" "Indices = 15"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.105334715" "Concs = 0.177716074" "Concs = 0.215668236"
## [7] "Concs = 0.273065521" "Concs = 0.362248011" "Concs = 0.403265809"
## [10] "Concs = 0.502168642" "Concs = 0.64289193" "Concs = 0.720570734"
## [13] "Concs = 0.725802164" "Concs = 0.865500124" "Concs = 0.956691847"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.028620 0.009439
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.15230 -0.03367 -0.01260 0.04921 0.12563
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.0286203 0.0312329 0.916 0.376
## Time[ind] 0.0094387 0.0006167 15.305 1.08e-09 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.07717 on 13 degrees of freedom
## Multiple R-squared: 0.9474, Adjusted R-squared: 0.9434
## F-statistic: 234.3 on 1 and 13 DF, p-value: 1.076e-09
## (Intercept) Time[ind]
## 0.028620334 0.009438665
## 2.5 % 97.5 %
## (Intercept) -0.038854153 0.09609482
## Time[ind] 0.008106383 0.01077095
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 10"
## [1] "Indices = 16" "Indices = 17" "Indices = 18" "Indices = 19"
## [5] "Indices = 20" "Indices = 21" "Indices = 22" "Indices = 23"
## [9] "Indices = 24" "Indices = 25" "Indices = 26" "Indices = 27"
## [13] "Indices = 28" "Indices = 29" "Indices = 30"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 60" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.062527948" "Concs = 0.066722432" "Concs = 0.073739184"
## [7] "Concs = 0.13417786" "Concs = 0.142144514" "Concs = 0.153899766"
## [10] "Concs = 0.359398841" "Concs = 0.284549411" "Concs = 0.293488967"
## [13] "Concs = 0.291970799" "Concs = 0.378114427" "Concs = 0.405964967"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.004784 0.004640
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.044227 -0.008723 -0.004784 0.005104 0.076243
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.004784 0.010614 0.451 0.66
## Time[ind] 0.004640 0.000223 20.804 2.31e-11 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.02585 on 13 degrees of freedom
## Multiple R-squared: 0.9708, Adjusted R-squared: 0.9686
## F-statistic: 432.8 on 1 and 13 DF, p-value: 2.309e-11
## (Intercept) Time[ind]
## 0.004784043 0.004639529
## 2.5 % 97.5 %
## (Intercept) -0.018145174 0.027713259
## Time[ind] 0.004157742 0.005121316
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 100"
## [1] "Indices = 31" "Indices = 32" "Indices = 33" "Indices = 34"
## [5] "Indices = 35" "Indices = 36" "Indices = 37" "Indices = 38"
## [9] "Indices = 39" "Indices = 40" "Indices = 41" "Indices = 42"
## [13] "Indices = 43" "Indices = 44" "Indices = 45"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.692469949" "Concs = 0.598237597" "Concs = 0.603481745"
## [7] "Concs = 1.23886552" "Concs = 1.156190328" "Concs = 1.011651128"
## [10] "Concs = 2.056702931" "Concs = 1.850305243" "Concs = 1.516060546"
## [13] "Concs = 2.637550555" "Concs = 2.271829323" "Concs = 2.15464298"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.19461 0.02542
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.32748 -0.19461 0.02762 0.14305 0.33708
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.194607 0.086818 2.242 0.0431 *
## Time[ind] 0.025417 0.001714 14.827 1.59e-09 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.2145 on 13 degrees of freedom
## Multiple R-squared: 0.9442, Adjusted R-squared: 0.9399
## F-statistic: 219.8 on 1 and 13 DF, p-value: 1.593e-09
## (Intercept) Time[ind]
## 0.1946066 0.0254169
## 2.5 % 97.5 %
## (Intercept) 0.007046883 0.38216639
## Time[ind] 0.021713543 0.02912026
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 75"
## [1] "Indices = 46" "Indices = 47" "Indices = 48" "Indices = 49"
## [5] "Indices = 50" "Indices = 51" "Indices = 52" "Indices = 53"
## [9] "Indices = 54" "Indices = 55" "Indices = 56" "Indices = 57"
## [13] "Indices = 58" "Indices = 59" "Indices = 60"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.126910879" "Concs = 0.194298986" "Concs = 0.205689666"
## [7] "Concs = 0.343048244" "Concs = 0.344665429" "Concs = 0.433300567"
## [10] "Concs = 0.79823473" "Concs = 0.910129645" "Concs = 0.789753599"
## [13] "Concs = 1.097803094" "Concs = 1.250836941" "Concs = 0.891346428"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.006856 0.012450
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.235999 -0.032614 -0.006856 0.040146 0.156281
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.0068560 0.0371784 0.184 0.857
## Time[ind] 0.0124499 0.0007341 16.960 3.01e-10 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.09186 on 13 degrees of freedom
## Multiple R-squared: 0.9568, Adjusted R-squared: 0.9534
## F-statistic: 287.6 on 1 and 13 DF, p-value: 3.008e-10
## (Intercept) Time[ind]
## 0.00685602 0.01244988
## 2.5 % 97.5 %
## (Intercept) -0.07346298 0.08717502
## Time[ind] 0.01086398 0.01403577
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 125"
## [1] "Indices = 61" "Indices = 62" "Indices = 63" "Indices = 64"
## [5] "Indices = 65" "Indices = 66" "Indices = 67" "Indices = 68"
## [9] "Indices = 69" "Indices = 70" "Indices = 71" "Indices = 72"
## [13] "Indices = 73"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 60" "Times = 60"
## [11] "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.660583406" "Concs = 0.521827731" "Concs = 0.519014334"
## [7] "Concs = 0.937060708" "Concs = 0.947234926" "Concs = 1.586433592"
## [10] "Concs = 1.701961252" "Concs = 2.098184546" "Concs = 2.114078113"
## [13] "Concs = 1.9947066"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.15067 0.02247
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.1782 -0.1507 0.0313 0.1123 0.2031
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.150670 0.058446 2.578 0.0257 *
## Time[ind] 0.022469 0.001143 19.654 6.44e-10 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.1406 on 11 degrees of freedom
## Multiple R-squared: 0.9723, Adjusted R-squared: 0.9698
## F-statistic: 386.3 on 1 and 11 DF, p-value: 6.445e-10
## (Intercept) Time[ind]
## 0.15066966 0.02246945
## 2.5 % 97.5 %
## (Intercept) 0.02203109 0.27930823
## Time[ind] 0.01995315 0.02498576
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 0.712"
## [1] "Indices = 74" "Indices = 75" "Indices = 76" "Indices = 77"
## [5] "Indices = 78" "Indices = 79" "Indices = 80" "Indices = 81"
## [9] "Indices = 82" "Indices = 83" "Indices = 84" "Indices = 85"
## [13] "Indices = 86" "Indices = 87"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0"
## [6] "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0"
## [11] "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0 0
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## 0 0 0 0 0
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0 0 NA NA
## Time[ind] 0 0 NA NA
##
## Residual standard error: 0 on 12 degrees of freedom
## Multiple R-squared: NaN, Adjusted R-squared: NaN
## F-statistic: NaN on 1 and 12 DF, p-value: NA
## (Intercept) Time[ind]
## 0 0
## 2.5 % 97.5 %
## (Intercept) 0 0
## Time[ind] 0 0
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 3.1684"
## [1] "Indices = 88" "Indices = 89" "Indices = 90" "Indices = 91"
## [5] "Indices = 92" "Indices = 93" "Indices = 94" "Indices = 95"
## [9] "Indices = 96" "Indices = 97" "Indices = 98" "Indices = 99"
## [13] "Indices = 100" "Indices = 101" "Indices = 102"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0" "Concs = 0" "Concs = 0"
## [7] "Concs = 0" "Concs = 0" "Concs = 0"
## [10] "Concs = 0.004276181" "Concs = 0" "Concs = 0"
## [13] "Concs = 0.068958259" "Concs = 0.095246481" "Concs = 0.042564751"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## -0.0124160 0.0006791
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.028331 -0.007958 0.002229 0.012416 0.046542
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) -0.0124160 0.0082982 -1.496 0.15847
## Time[ind] 0.0006791 0.0001638 4.145 0.00115 **
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.0205 on 13 degrees of freedom
## Multiple R-squared: 0.5692, Adjusted R-squared: 0.5361
## F-statistic: 17.18 on 1 and 13 DF, p-value: 0.001152
## (Intercept) Time[ind]
## -0.0124160299 0.0006791216
## 2.5 % 97.5 %
## (Intercept) -0.0303432318 0.005511172
## Time[ind] 0.0003251496 0.001033094
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 200"
## [1] "Indices = 103" "Indices = 104" "Indices = 105" "Indices = 106"
## [5] "Indices = 107" "Indices = 108" "Indices = 109" "Indices = 110"
## [9] "Indices = 111" "Indices = 112" "Indices = 113" "Indices = 114"
## [13] "Indices = 115" "Indices = 116" "Indices = 117"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0" "Concs = 0" "Concs = 0"
## [7] "Concs = 0" "Concs = 0" "Concs = 0"
## [10] "Concs = 0" "Concs = 0.08814803" "Concs = 0"
## [13] "Concs = 0.636612173" "Concs = 0.308057976" "Concs = 0.343301822"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## -0.076456 0.004313
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.18231 -0.05293 0.01176 0.05403 0.32492
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) -0.076456 0.051227 -1.492 0.159438
## Time[ind] 0.004313 0.001011 4.264 0.000923 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.1266 on 13 degrees of freedom
## Multiple R-squared: 0.5831, Adjusted R-squared: 0.551
## F-statistic: 18.18 on 1 and 13 DF, p-value: 0.0009232
## (Intercept) Time[ind]
## -0.076455952 0.004312751
## 2.5 % 97.5 %
## (Intercept) -0.187125601 0.034213697
## Time[ind] 0.002127583 0.006497919
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 250"
## [1] "Indices = 118" "Indices = 119" "Indices = 120" "Indices = 121"
## [5] "Indices = 122" "Indices = 123" "Indices = 124" "Indices = 125"
## [9] "Indices = 126" "Indices = 127" "Indices = 128" "Indices = 129"
## [13] "Indices = 130"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 60" "Times = 60"
## [11] "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.303186347" "Concs = 0.314346484" "Concs = 0.32890249"
## [7] "Concs = 0.470955087" "Concs = 0.418661875" "Concs = 0.69686814"
## [10] "Concs = 0.65732937" "Concs = 1.058297138" "Concs = 1.054151944"
## [13] "Concs = 0.826804017"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.08568 0.01013
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.17082 -0.08568 0.02900 0.06552 0.09123
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.0856770 0.0366138 2.34 0.0392 *
## Time[ind] 0.0101327 0.0007162 14.15 2.11e-08 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.08809 on 11 degrees of freedom
## Multiple R-squared: 0.9479, Adjusted R-squared: 0.9432
## F-statistic: 200.2 on 1 and 11 DF, p-value: 2.106e-08
## (Intercept) Time[ind]
## 0.08567696 0.01013273
## 2.5 % 97.5 %
## (Intercept) 0.005090447 0.16626348
## Time[ind] 0.008556376 0.01170909
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 150"
## [1] "Indices = 131" "Indices = 132" "Indices = 133" "Indices = 134"
## [5] "Indices = 135" "Indices = 136" "Indices = 137" "Indices = 138"
## [9] "Indices = 139" "Indices = 140" "Indices = 141" "Indices = 142"
## [13] "Indices = 143" "Indices = 144" "Indices = 145" "Indices = 146"
## [17] "Indices = 147" "Indices = 148"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 0" "Times = 15"
## [6] "Times = 15" "Times = 15" "Times = 15" "Times = 30" "Times = 30"
## [11] "Times = 30" "Times = 30" "Times = 60" "Times = 60" "Times = 60"
## [16] "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0" "Concs = 0.651134538" "Concs = 0.410418033"
## [7] "Concs = 0.72633168" "Concs = 0.655344167" "Concs = 1.213331632"
## [10] "Concs = 0.938854783" "Concs = 1.336588359" "Concs = 1.220393789"
## [13] "Concs = 2.258618197" "Concs = 2.185187246" "Concs = 2.037689206"
## [16] "Concs = 2.794152863" "Concs = 2.97663412" "Concs = 2.702787847"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.12001 0.03166
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.26683 -0.12001 0.01248 0.14043 0.26671
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.120012 0.058315 2.058 0.0563 .
## Time[ind] 0.031662 0.001243 25.467 2.24e-14 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.1647 on 16 degrees of freedom
## Multiple R-squared: 0.9759, Adjusted R-squared: 0.9744
## F-statistic: 648.6 on 1 and 16 DF, p-value: 2.241e-14
## (Intercept) Time[ind]
## 0.12001167 0.03166231
## 2.5 % 97.5 %
## (Intercept) -0.003610993 0.24363433
## Time[ind] 0.029026668 0.03429796
## [1] "##################################################"## 'data.frame': 10 obs. of 2 variables:
## $ sub: num 50 10 100 75 125 ...
## $ vel: num 47.2 23.2 127.1 62.2 112.3 ...## sub vel
## 1 50.0000 47.193323
## 2 10.0000 23.197644
## 3 100.0000 127.084516
## 4 75.0000 62.249384
## 5 125.0000 112.347269
## 6 0.7120 0.000000
## 7 3.1684 3.395608
## 8 200.0000 21.563755
## 9 250.0000 50.663660
## 10 150.0000 158.311559
We are interested in the reaction rate, v, which is a function of available substrate (pesticide) and the presence of an enzyme. We get Vmax from the asymptote and K = 0.5*Vmax. Biochemical reactions with a single substrate are typically assumed to follow Michaelis-Menten kinetics even if they deviate from the basic model assumptions.
We are fitting the 2-parameter model.
Used to estimate slopes.
dia
The above figure shows a three-dimensional plot of pesticide substrate concentration, observed concentration and experiment time. We need to condition the pesticide data on the substrate concentrations used in the experiment and regress the observed concentrations over the 90 min period in order to estimate the linear slopes for the Michaelis-Menten kinetic rate constants.
## [1] "Substrates:"## [1] "##################################################"
## [1] "Substrate = 50"
## [1] "Indices = 1" "Indices = 2" "Indices = 3" "Indices = 4"
## [5] "Indices = 5" "Indices = 6" "Indices = 7" "Indices = 8"
## [9] "Indices = 9" "Indices = 10" "Indices = 11" "Indices = 12"
## [13] "Indices = 13" "Indices = 14" "Indices = 15"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.17588934" "Concs = 0.162027282" "Concs = 0.163356559"
## [7] "Concs = 0.343213005" "Concs = 0.307927387" "Concs = 0.309459105"
## [10] "Concs = 0.560097677" "Concs = 0.54821645" "Concs = 0.533109282"
## [13] "Concs = 0.73827321" "Concs = 0.728694143" "Concs = 0.726811372"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.040150 0.008025
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.040150 -0.034677 0.002826 0.026782 0.062303
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.0401505 0.0141144 2.845 0.0138 *
## Time[ind] 0.0080253 0.0002787 28.797 3.67e-13 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.03488 on 13 degrees of freedom
## Multiple R-squared: 0.9846, Adjusted R-squared: 0.9834
## F-statistic: 829.3 on 1 and 13 DF, p-value: 3.671e-13
## (Intercept) Time[ind]
## 0.040150483 0.008025329
## 2.5 % 97.5 %
## (Intercept) 0.009658192 0.070642775
## Time[ind] 0.007423260 0.008627398
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 10"
## [1] "Indices = 16" "Indices = 17" "Indices = 18" "Indices = 19"
## [5] "Indices = 20" "Indices = 21" "Indices = 22" "Indices = 23"
## [9] "Indices = 24" "Indices = 25" "Indices = 26" "Indices = 27"
## [13] "Indices = 28" "Indices = 29" "Indices = 30"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 60" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.037225886" "Concs = 0.038369499" "Concs = 0.046312162"
## [7] "Concs = 0.08490015" "Concs = 0.083838648" "Concs = 0.093345378"
## [10] "Concs = 0.209721193" "Concs = 0.168743119" "Concs = 0.161851994"
## [13] "Concs = 0.162150446" "Concs = 0.222852555" "Concs = 0.229604672"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.004532 0.002650
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.020209 -0.005225 -0.001700 0.001442 0.046169
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.0045322 0.0062267 0.728 0.48
## Time[ind] 0.0026503 0.0001308 20.257 3.23e-11 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.01517 on 13 degrees of freedom
## Multiple R-squared: 0.9693, Adjusted R-squared: 0.9669
## F-statistic: 410.3 on 1 and 13 DF, p-value: 3.233e-11
## (Intercept) Time[ind]
## 0.004532231 0.002650328
## 2.5 % 97.5 %
## (Intercept) -0.008919788 0.017984250
## Time[ind] 0.002367676 0.002932981
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 100"
## [1] "Indices = 31" "Indices = 32" "Indices = 33" "Indices = 34"
## [5] "Indices = 35" "Indices = 36" "Indices = 37" "Indices = 38"
## [9] "Indices = 39" "Indices = 40" "Indices = 41" "Indices = 42"
## [13] "Indices = 43" "Indices = 44" "Indices = 45"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.519984026" "Concs = 0.456185794" "Concs = 0.465640368"
## [7] "Concs = 0.969762496" "Concs = 0.925948373" "Concs = 0.828633383"
## [10] "Concs = 1.646101156" "Concs = 1.493978674" "Concs = 1.273473826"
## [13] "Concs = 2.097846916" "Concs = 1.845660879" "Concs = 1.775884862"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.14326 0.02077
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.23664 -0.14326 0.01084 0.09494 0.25667
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.14326 0.06330 2.263 0.0414 *
## Time[ind] 0.02077 0.00125 16.617 3.88e-10 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.1564 on 13 degrees of freedom
## Multiple R-squared: 0.955, Adjusted R-squared: 0.9516
## F-statistic: 276.1 on 1 and 13 DF, p-value: 3.88e-10
## (Intercept) Time[ind]
## 0.14326104 0.02076955
## 2.5 % 97.5 %
## (Intercept) 0.006505081 0.28001700
## Time[ind] 0.018069306 0.02346979
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 75"
## [1] "Indices = 46" "Indices = 47" "Indices = 48" "Indices = 49"
## [5] "Indices = 50" "Indices = 51" "Indices = 52" "Indices = 53"
## [9] "Indices = 54" "Indices = 55" "Indices = 56" "Indices = 57"
## [13] "Indices = 58" "Indices = 59" "Indices = 60"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.105167732" "Concs = 0.164272494" "Concs = 0.177858813"
## [7] "Concs = 0.289730134" "Concs = 0.302066953" "Concs = 0.387892352"
## [10] "Concs = 0.703975509" "Concs = 0.8027739" "Concs = 0.718751156"
## [13] "Concs = 0.974859321" "Concs = 1.104369407" "Concs = 0.769135715"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.003918 0.011012
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.225872 -0.026182 -0.003918 0.046471 0.138129
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.0039178 0.0349716 0.112 0.913
## Time[ind] 0.0110121 0.0006905 15.948 6.47e-10 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.08641 on 13 degrees of freedom
## Multiple R-squared: 0.9514, Adjusted R-squared: 0.9476
## F-statistic: 254.3 on 1 and 13 DF, p-value: 6.468e-10
## (Intercept) Time[ind]
## 0.003917758 0.011012115
## 2.5 % 97.5 %
## (Intercept) -0.071633704 0.07946922
## Time[ind] 0.009520354 0.01250388
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 125"
## [1] "Indices = 61" "Indices = 62" "Indices = 63" "Indices = 64"
## [5] "Indices = 65" "Indices = 66" "Indices = 67" "Indices = 68"
## [9] "Indices = 69" "Indices = 70" "Indices = 71" "Indices = 72"
## [13] "Indices = 73"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 60" "Times = 60"
## [11] "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.558156141" "Concs = 0.453398653" "Concs = 0.457441572"
## [7] "Concs = 0.819739976" "Concs = 0.818340148" "Concs = 1.39707951"
## [10] "Concs = 1.483198815" "Concs = 1.818559552" "Concs = 1.850457042"
## [13] "Concs = 1.741234977"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.12932 0.01963
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.15472 -0.12932 0.02964 0.10015 0.17613
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.1293156 0.0503960 2.566 0.0262 *
## Time[ind] 0.0196293 0.0009858 19.912 5.6e-10 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.1212 on 11 degrees of freedom
## Multiple R-squared: 0.973, Adjusted R-squared: 0.9706
## F-statistic: 396.5 on 1 and 11 DF, p-value: 5.603e-10
## (Intercept) Time[ind]
## 0.1293156 0.0196293
## 2.5 % 97.5 %
## (Intercept) 0.01839473 0.24023649
## Time[ind] 0.01745957 0.02179903
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 0.712"
## [1] "Indices = 74" "Indices = 75" "Indices = 76" "Indices = 77"
## [5] "Indices = 78" "Indices = 79" "Indices = 80" "Indices = 81"
## [9] "Indices = 82" "Indices = 83" "Indices = 84" "Indices = 85"
## [13] "Indices = 86" "Indices = 87" "Indices = 88"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0"
## [6] "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0"
## [11] "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0 0
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## 0 0 0 0 0
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0 0 NA NA
## Time[ind] 0 0 NA NA
##
## Residual standard error: 0 on 13 degrees of freedom
## Multiple R-squared: NaN, Adjusted R-squared: NaN
## F-statistic: NaN on 1 and 13 DF, p-value: NA
## (Intercept) Time[ind]
## 0 0
## 2.5 % 97.5 %
## (Intercept) 0 0
## Time[ind] 0 0
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 3.1684"
## [1] "Indices = 89" "Indices = 90" "Indices = 91" "Indices = 92"
## [5] "Indices = 93" "Indices = 94" "Indices = 95" "Indices = 96"
## [9] "Indices = 97" "Indices = 98" "Indices = 99" "Indices = 100"
## [13] "Indices = 101" "Indices = 102" "Indices = 103"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0"
## [6] "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0"
## [11] "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0" "Concs = 0"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0 0
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## 0 0 0 0 0
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0 0 NA NA
## Time[ind] 0 0 NA NA
##
## Residual standard error: 0 on 13 degrees of freedom
## Multiple R-squared: NaN, Adjusted R-squared: NaN
## F-statistic: NaN on 1 and 13 DF, p-value: NA
## (Intercept) Time[ind]
## 0 0
## 2.5 % 97.5 %
## (Intercept) 0 0
## Time[ind] 0 0
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 250"
## [1] "Indices = 104" "Indices = 105" "Indices = 106" "Indices = 107"
## [5] "Indices = 108" "Indices = 109" "Indices = 110" "Indices = 111"
## [9] "Indices = 112" "Indices = 113" "Indices = 114" "Indices = 115"
## [13] "Indices = 116"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 60" "Times = 60"
## [11] "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.488073154" "Concs = 0.494203709" "Concs = 0.58569046"
## [7] "Concs = 0.731173258" "Concs = 0.714667917" "Concs = 1.145221545"
## [10] "Concs = 1.154181588" "Concs = 1.638731263" "Concs = 1.563749853"
## [13] "Concs = 1.475092589"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.15075 0.01622
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.15075 -0.13584 0.02780 0.09369 0.19157
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.1507544 0.0503259 2.996 0.0122 *
## Time[ind] 0.0162242 0.0009844 16.481 4.21e-09 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.1211 on 11 degrees of freedom
## Multiple R-squared: 0.9611, Adjusted R-squared: 0.9575
## F-statistic: 271.6 on 1 and 11 DF, p-value: 4.214e-09
## (Intercept) Time[ind]
## 0.1507544 0.0162242
## 2.5 % 97.5 %
## (Intercept) 0.03998791 0.26152088
## Time[ind] 0.01405749 0.01839091
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 150"
## [1] "Indices = 117" "Indices = 118" "Indices = 119" "Indices = 120"
## [5] "Indices = 121" "Indices = 122" "Indices = 123" "Indices = 124"
## [9] "Indices = 125" "Indices = 126" "Indices = 127" "Indices = 128"
## [13] "Indices = 129" "Indices = 130" "Indices = 131" "Indices = 132"
## [17] "Indices = 133" "Indices = 134"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 0" "Times = 15"
## [6] "Times = 15" "Times = 15" "Times = 15" "Times = 30" "Times = 30"
## [11] "Times = 30" "Times = 30" "Times = 60" "Times = 60" "Times = 60"
## [16] "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0" "Concs = 0.462309631" "Concs = 0.255700106"
## [7] "Concs = 0.526463046" "Concs = 0.47230486" "Concs = 0.931548447"
## [10] "Concs = 0.722337549" "Concs = 1.066740758" "Concs = 0.948249061"
## [13] "Concs = 1.827879364" "Concs = 1.789768545" "Concs = 1.614786398"
## [16] "Concs = 2.243971727" "Concs = 2.385364241" "Concs = 2.16439478"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.06633 0.02574
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.21878 -0.06632 0.00304 0.08820 0.22813
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.066325 0.048063 1.38 0.187
## Time[ind] 0.025743 0.001025 25.12 2.77e-14 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.1357 on 16 degrees of freedom
## Multiple R-squared: 0.9753, Adjusted R-squared: 0.9737
## F-statistic: 631.1 on 1 and 16 DF, p-value: 2.773e-14
## (Intercept) Time[ind]
## 0.0663253 0.0257428
## 2.5 % 97.5 %
## (Intercept) -0.03556328 0.16821388
## Time[ind] 0.02357053 0.02791507
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 200"
## [1] "Indices = 135" "Indices = 136" "Indices = 137" "Indices = 138"
## [5] "Indices = 139" "Indices = 140" "Indices = 141" "Indices = 142"
## [9] "Indices = 143" "Indices = 144" "Indices = 145" "Indices = 146"
## [13] "Indices = 147" "Indices = 148" "Indices = 149"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0" "Concs = 0" "Concs = 0"
## [7] "Concs = 0" "Concs = 0" "Concs = 0"
## [10] "Concs = 0.147179292" "Concs = 0.49374336" "Concs = 0.185151589"
## [13] "Concs = 0.905020781" "Concs = 0.751527127" "Concs = 0.778803003"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## -0.135092 0.009039
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.260068 -0.136078 -0.000493 0.117739 0.226604
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) -0.135092 0.061636 -2.192 0.0472 *
## Time[ind] 0.009039 0.001217 7.427 4.99e-06 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.1523 on 13 degrees of freedom
## Multiple R-squared: 0.8093, Adjusted R-squared: 0.7946
## F-statistic: 55.16 on 1 and 13 DF, p-value: 4.994e-06
## (Intercept) Time[ind]
## -0.135091948 0.009038982
## 2.5 % 97.5 %
## (Intercept) -0.268247657 -0.001936239
## Time[ind] 0.006409827 0.011668136
## [1] "##################################################"## 'data.frame': 10 obs. of 2 variables:
## $ sub: num 50 10 100 75 125 ...
## $ vel: num 40.1 13.3 103.8 55.1 98.1 ...## sub vel
## 1 50.0000 40.12665
## 2 10.0000 13.25164
## 3 100.0000 103.84774
## 4 75.0000 55.06057
## 5 125.0000 98.14650
## 6 0.7120 0.00000
## 7 3.1684 0.00000
## 8 250.0000 81.12099
## 9 150.0000 128.71399
## 10 200.0000 45.19491
triadimefon
The above figure shows a three-dimensional plot of pesticide substrate concentration, observed concentration and experiment time. We need to condition the pesticide data on the substrate concentrations used in the experiment and regress the observed concentrations over the 90 min period in order to estimate the linear slopes for the Michaelis-Menten kinetic rate constants.
## [1] "Substrates:"## [1] "##################################################"
## [1] "Substrate = 0.68082"
## [1] "Indices = 1" "Indices = 2" "Indices = 3" "Indices = 4"
## [5] "Indices = 5" "Indices = 6" "Indices = 7" "Indices = 8"
## [9] "Indices = 9" "Indices = 10" "Indices = 11" "Indices = 12"
## [13] "Indices = 13" "Indices = 14" "Indices = 15"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0.491983075" "Concs = 0.401196591" "Concs = 0.426789449"
## [4] "Concs = 0.399443695" "Concs = 0.42224169" "Concs = 0.36799564"
## [7] "Concs = 0.335043095" "Concs = 0.307545884" "Concs = 0.33049829"
## [10] "Concs = 0.298781298" "Concs = 0.300851183" "Concs = 0.297372843"
## [13] "Concs = 0.30988245" "Concs = 0.29724505" "Concs = 0.293730776"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.411830 -0.001533
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.05829 -0.02177 -0.01063 0.02164 0.08015
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.4118302 0.0148783 27.680 6.09e-13 ***
## Time[ind] -0.0015331 0.0002938 -5.219 0.000166 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.03676 on 13 degrees of freedom
## Multiple R-squared: 0.6769, Adjusted R-squared: 0.652
## F-statistic: 27.23 on 1 and 13 DF, p-value: 0.0001656
## (Intercept) Time[ind]
## 0.411830209 -0.001533081
## 2.5 % 97.5 %
## (Intercept) 0.379687608 0.4439728100
## Time[ind] -0.002167735 -0.0008984261
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 10"
## [1] "Indices = 16" "Indices = 17" "Indices = 18" "Indices = 19"
## [5] "Indices = 20" "Indices = 21" "Indices = 22" "Indices = 23"
## [9] "Indices = 24" "Indices = 25" "Indices = 26" "Indices = 27"
## [13] "Indices = 28" "Indices = 29"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 1.66496797" "Concs = 1.809450976" "Concs = 2.047584443"
## [4] "Concs = 3.796829723" "Concs = 3.995422538" "Concs = 4.084402023"
## [7] "Concs = 3.689670522" "Concs = 3.770627455" "Concs = 3.708045842"
## [10] "Concs = 2.923805377" "Concs = 2.81999575" "Concs = 1.627429272"
## [13] "Concs = 1.556106647" "Concs = 1.615639352"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 3.23029 -0.01165
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -1.5653 -0.6112 0.3404 0.8225 1.0288
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 3.230286 0.400694 8.062 3.48e-06 ***
## Time[ind] -0.011646 0.008028 -1.451 0.173
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.9894 on 12 degrees of freedom
## Multiple R-squared: 0.1492, Adjusted R-squared: 0.07829
## F-statistic: 2.104 on 1 and 12 DF, p-value: 0.1725
## (Intercept) Time[ind]
## 3.23028639 -0.01164577
## 2.5 % 97.5 %
## (Intercept) 2.35724967 4.103323110
## Time[ind] -0.02913777 0.005846224
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 2.89"
## [1] "Indices = 30" "Indices = 31" "Indices = 32" "Indices = 33"
## [5] "Indices = 34" "Indices = 35" "Indices = 36" "Indices = 37"
## [9] "Indices = 38" "Indices = 39" "Indices = 40" "Indices = 41"
## [13] "Indices = 42" "Indices = 43" "Indices = 44"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0.953124823" "Concs = 0.851227037" "Concs = 0.735830293"
## [4] "Concs = 1.438977536" "Concs = 1.147878572" "Concs = 1.273836719"
## [7] "Concs = 1.170628317" "Concs = 1.153347335" "Concs = 0.90609785"
## [10] "Concs = 0.796555881" "Concs = 0.724314066" "Concs = 0.705525399"
## [13] "Concs = 0.684015583" "Concs = 0.645877009" "Concs = 0.633912406"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 1.105417 -0.004718
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.36959 -0.10741 -0.03491 0.15135 0.40433
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 1.105417 0.084540 13.076 7.41e-09 ***
## Time[ind] -0.004718 0.001669 -2.827 0.0143 *
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.2089 on 13 degrees of freedom
## Multiple R-squared: 0.3806, Adjusted R-squared: 0.333
## F-statistic: 7.989 on 1 and 13 DF, p-value: 0.01429
## (Intercept) Time[ind]
## 1.105417292 -0.004718138
## 2.5 % 97.5 %
## (Intercept) 0.922779946 1.28805464
## Time[ind] -0.008324305 -0.00111197
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 50"
## [1] "Indices = 45" "Indices = 46" "Indices = 47" "Indices = 48"
## [5] "Indices = 49" "Indices = 50" "Indices = 51" "Indices = 52"
## [9] "Indices = 53" "Indices = 54" "Indices = 55" "Indices = 56"
## [13] "Indices = 57"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 60" "Times = 60"
## [11] "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 24.40802225" "Concs = 24.82657815" "Concs = 23.72724558"
## [4] "Concs = 25.6223204" "Concs = 24.15898057" "Concs = 24.97516518"
## [7] "Concs = 26.62994332" "Concs = 26.25228145" "Concs = 25.95782864"
## [10] "Concs = 28.59868326" "Concs = 22.32352998" "Concs = 22.61126729"
## [13] "Concs = 22.93204565"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 25.37794 -0.01392
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -1.8018 -1.1933 -0.5514 1.2919 4.0558
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 25.37794 0.74253 34.178 1.61e-12 ***
## Time[ind] -0.01392 0.01452 -0.958 0.359
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 1.786 on 11 degrees of freedom
## Multiple R-squared: 0.07704, Adjusted R-squared: -0.006863
## F-statistic: 0.9182 on 1 and 11 DF, p-value: 0.3585
## (Intercept) Time[ind]
## 25.37794389 -0.01391794
## 2.5 % 97.5 %
## (Intercept) 23.74364659 27.01224120
## Time[ind] -0.04588649 0.01805062
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 75"
## [1] "Indices = 58" "Indices = 59" "Indices = 60" "Indices = 61"
## [5] "Indices = 62" "Indices = 63" "Indices = 64" "Indices = 65"
## [9] "Indices = 66" "Indices = 67" "Indices = 68" "Indices = 69"
## [13] "Indices = 70" "Indices = 71" "Indices = 72"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 40.78378417" "Concs = 39.14533446" "Concs = 37.19980911"
## [4] "Concs = 39.60290919" "Concs = 36.37647057" "Concs = 38.41537648"
## [7] "Concs = 41.36228948" "Concs = 39.60347726" "Concs = 37.05197615"
## [10] "Concs = 38.79077057" "Concs = 39.14694989" "Concs = 38.88285882"
## [13] "Concs = 38.84162326" "Concs = 38.38496898" "Concs = 38.08875118"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 38.88146 -0.00264
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -2.4654 -0.4908 0.1598 0.5925 2.5600
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 38.88146 0.55263 70.357 <2e-16 ***
## Time[ind] -0.00264 0.01091 -0.242 0.813
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 1.365 on 13 degrees of freedom
## Multiple R-squared: 0.004484, Adjusted R-squared: -0.07209
## F-statistic: 0.05855 on 1 and 13 DF, p-value: 0.8126
## (Intercept) Time[ind]
## 38.881462270 -0.002640315
## 2.5 % 97.5 %
## (Intercept) 37.68758232 40.0753422
## Time[ind] -0.02621343 0.0209328
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 100"
## [1] "Indices = 73" "Indices = 74" "Indices = 75" "Indices = 76"
## [5] "Indices = 77" "Indices = 78" "Indices = 79" "Indices = 80"
## [9] "Indices = 81" "Indices = 82" "Indices = 83" "Indices = 84"
## [13] "Indices = 85" "Indices = 86" "Indices = 87"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 48.8233658" "Concs = 45.64682255" "Concs = 49.68642703"
## [4] "Concs = 49.24893567" "Concs = 52.23999837" "Concs = 54.94800062"
## [7] "Concs = 51.63283427" "Concs = 55.62979138" "Concs = 53.25296347"
## [10] "Concs = 57.2285182" "Concs = 52.25557462" "Concs = 57.56142454"
## [13] "Concs = 55.15134637" "Concs = 52.80788424" "Concs = 54.77096052"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 50.27020 0.06296
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -4.6234 -1.6195 -0.5838 2.1373 3.7334
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 50.27020 1.08609 46.286 8.14e-16 ***
## Time[ind] 0.06296 0.02144 2.936 0.0116 *
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 2.684 on 13 degrees of freedom
## Multiple R-squared: 0.3987, Adjusted R-squared: 0.3524
## F-statistic: 8.62 on 1 and 13 DF, p-value: 0.01158
## (Intercept) Time[ind]
## 50.27019838 0.06296046
## 2.5 % 97.5 %
## (Intercept) 47.92384633 52.6165504
## Time[ind] 0.01663183 0.1092891
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 125"
## [1] "Indices = 88" "Indices = 89" "Indices = 90" "Indices = 91"
## [5] "Indices = 92" "Indices = 93" "Indices = 94" "Indices = 95"
## [9] "Indices = 96" "Indices = 97" "Indices = 98" "Indices = 99"
## [13] "Indices = 100" "Indices = 101"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 61.59837616" "Concs = 58.92902388" "Concs = 62.18995319"
## [4] "Concs = 54.97761564" "Concs = 64.19295623" "Concs = 61.97688614"
## [7] "Concs = 68.89112849" "Concs = 56.33566744" "Concs = 71.31751913"
## [10] "Concs = 66.04862513" "Concs = 69.71262134" "Concs = 57.48171016"
## [13] "Concs = 60.79276739" "Concs = 57.42314849"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 62.580107 -0.008102
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -7.4810 -4.1897 -0.7317 3.3996 8.9805
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 62.580107 2.177110 28.745 1.95e-12 ***
## Time[ind] -0.008102 0.043620 -0.186 0.856
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 5.376 on 12 degrees of freedom
## Multiple R-squared: 0.002867, Adjusted R-squared: -0.08023
## F-statistic: 0.0345 on 1 and 12 DF, p-value: 0.8558
## (Intercept) Time[ind]
## 62.580106621 -0.008101893
## 2.5 % 97.5 %
## (Intercept) 57.8365915 67.32362176
## Time[ind] -0.1031421 0.08693828
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 150"
## [1] "Indices = 102" "Indices = 103" "Indices = 104" "Indices = 105"
## [5] "Indices = 106" "Indices = 107" "Indices = 108" "Indices = 109"
## [9] "Indices = 110" "Indices = 111" "Indices = 112" "Indices = 113"
## [13] "Indices = 114" "Indices = 115"
## [1] "Times = 0" "Times = 0" "Times = 15" "Times = 15" "Times = 15"
## [6] "Times = 30" "Times = 30" "Times = 30" "Times = 60" "Times = 60"
## [11] "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 65.77539856" "Concs = 67.99385172" "Concs = 78.63355587"
## [4] "Concs = 62.93987056" "Concs = 106.7018113" "Concs = 73.37624336"
## [7] "Concs = 66.9635187" "Concs = 95.5133834" "Concs = 69.7678651"
## [10] "Concs = 58.53815945" "Concs = 61.06336245" "Concs = 82.43537943"
## [13] "Concs = 75.76324133" "Concs = 73.77914496"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 75.38048 -0.02749
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -15.193 -9.102 -2.571 3.463 31.734
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 75.38048 6.14976 12.257 3.82e-08 ***
## Time[ind] -0.02749 0.11731 -0.234 0.819
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 13.9 on 12 degrees of freedom
## Multiple R-squared: 0.004555, Adjusted R-squared: -0.0784
## F-statistic: 0.05492 on 1 and 12 DF, p-value: 0.8187
## (Intercept) Time[ind]
## 75.38047797 -0.02749044
## 2.5 % 97.5 %
## (Intercept) 61.9813052 88.7796507
## Time[ind] -0.2830856 0.2281048
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 200"
## [1] "Indices = 116" "Indices = 117" "Indices = 118" "Indices = 119"
## [5] "Indices = 120" "Indices = 121" "Indices = 122" "Indices = 123"
## [9] "Indices = 124" "Indices = 125" "Indices = 126" "Indices = 127"
## [13] "Indices = 128" "Indices = 129" "Indices = 130"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 293.4352803" "Concs = 357.1944002" "Concs = 335.0321393"
## [4] "Concs = 338.7826757" "Concs = 357.9520843" "Concs = 202.0964578"
## [7] "Concs = 422.3552358" "Concs = 340.5253492" "Concs = 345.8291382"
## [10] "Concs = 359.4674526" "Concs = 377.2730298" "Concs = 186.1093226"
## [13] "Concs = 471.6047045" "Concs = 376.1365036" "Concs = 190.6933116"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 324.45 0.15
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -147.34 -10.22 16.88 35.47 133.66
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 324.4495 34.2770 9.466 3.39e-07 ***
## Time[ind] 0.1500 0.6768 0.222 0.828
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 84.69 on 13 degrees of freedom
## Multiple R-squared: 0.003764, Adjusted R-squared: -0.07287
## F-statistic: 0.04911 on 1 and 13 DF, p-value: 0.8281
## (Intercept) Time[ind]
## 324.449491 0.149991
## 2.5 % 97.5 %
## (Intercept) 250.398438 398.500544
## Time[ind] -1.312144 1.612126
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 250"
## [1] "Indices = 131" "Indices = 132" "Indices = 133" "Indices = 134"
## [5] "Indices = 135" "Indices = 136" "Indices = 137" "Indices = 138"
## [9] "Indices = 139" "Indices = 140" "Indices = 141" "Indices = 142"
## [13] "Indices = 143" "Indices = 144"
## [1] "Times = 0" "Times = 0" "Times = 15" "Times = 15" "Times = 15"
## [6] "Times = 30" "Times = 30" "Times = 30" "Times = 60" "Times = 60"
## [11] "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 222.4579985" "Concs = 180.8725406" "Concs = 186.8824662"
## [4] "Concs = 208.9457498" "Concs = 167.0779465" "Concs = 229.5166359"
## [7] "Concs = 99.11335239" "Concs = 263.0350796" "Concs = 59.52448054"
## [10] "Concs = 46.29054385" "Concs = 51.98182232" "Concs = 162.0360626"
## [13] "Concs = 200.5560551" "Concs = 187.8908429"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 190.1312 -0.6763
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -103.26 -56.27 17.93 52.17 93.19
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 190.1312 30.5866 6.216 4.48e-05 ***
## Time[ind] -0.6763 0.5835 -1.159 0.269
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 69.11 on 12 degrees of freedom
## Multiple R-squared: 0.1007, Adjusted R-squared: 0.02576
## F-statistic: 1.344 on 1 and 12 DF, p-value: 0.2689
## (Intercept) Time[ind]
## 190.131172 -0.676333
## 2.5 % 97.5 %
## (Intercept) 123.488657 256.7736868
## Time[ind] -1.947569 0.5949027
## [1] "##################################################"## 'data.frame': 10 obs. of 2 variables:
## $ sub: num 0.681 10 2.89 50 75 ...
## $ vel: num 7.67 58.23 23.59 69.59 13.2 ...## sub vel
## 1 0.68082 7.665403
## 2 10.00000 58.228872
## 3 2.89000 23.590688
## 4 50.00000 69.589686
## 5 75.00000 13.201577
## 6 100.00000 -314.802324
## 7 125.00000 40.509466
## 8 150.00000 137.452183
## 9 200.00000 -749.954886
## 10 250.00000 3381.665221
tdla
The above figure shows a three-dimensional plot of pesticide substrate concentration, observed concentration and experiment time. We need to condition the pesticide data on the substrate concentrations used in the experiment and regress the observed concentrations over the 90 min period in order to estimate the linear slopes for the Michaelis-Menten kinetic rate constants.
## [1] "Substrates:"## [1] "##################################################"
## [1] "Substrate = 0.68"
## [1] "Indices = 1" "Indices = 2" "Indices = 3" "Indices = 4"
## [5] "Indices = 5" "Indices = 6" "Indices = 7" "Indices = 8"
## [9] "Indices = 9" "Indices = 10" "Indices = 11" "Indices = 12"
## [13] "Indices = 13" "Indices = 14" "Indices = 15"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0.776869584" "Concs = 0.395237886" "Concs = 2.220388898"
## [4] "Concs = 0.124349504" "Concs = 0.14627783" "Concs = 0.089752887"
## [7] "Concs = 0.107638677" "Concs = 0.159434476" "Concs = 0.159787554"
## [10] "Concs = 0.099828091" "Concs = 0.225228369" "Concs = 0.139329405"
## [13] "Concs = 0.072482112" "Concs = 0.09994686" "Concs = 0.061506341"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.631813 -0.007862
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.42413 -0.26245 -0.06028 0.14115 1.58858
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.631813 0.205207 3.079 0.0088 **
## Time[ind] -0.007862 0.004052 -1.940 0.0743 .
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.507 on 13 degrees of freedom
## Multiple R-squared: 0.2246, Adjusted R-squared: 0.1649
## F-statistic: 3.765 on 1 and 13 DF, p-value: 0.07434
## (Intercept) Time[ind]
## 0.631813196 -0.007861777
## 2.5 % 97.5 %
## (Intercept) 0.18849145 1.0751349430
## Time[ind] -0.01661515 0.0008915944
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 10"
## [1] "Indices = 16" "Indices = 17" "Indices = 18" "Indices = 19"
## [5] "Indices = 20" "Indices = 21" "Indices = 22" "Indices = 23"
## [9] "Indices = 24" "Indices = 25" "Indices = 26" "Indices = 27"
## [13] "Indices = 28" "Indices = 29" "Indices = 30"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0.964686974" "Concs = 0.95171618" "Concs = 0.951349197"
## [4] "Concs = 0.562008549" "Concs = 0.514503123" "Concs = 0.553400833"
## [7] "Concs = 0.914781067" "Concs = 0.896944628" "Concs = 0.854682343"
## [10] "Concs = 0.640465968" "Concs = 1.46510307" "Concs = 1.529173181"
## [13] "Concs = 1.670981262" "Concs = 1.594325825" "Concs = 1.598291535"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.672054 0.009541
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.60406 -0.17839 0.06356 0.24994 0.29263
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.672054 0.112477 5.975 4.63e-05 ***
## Time[ind] 0.009541 0.002221 4.296 0.000869 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.2779 on 13 degrees of freedom
## Multiple R-squared: 0.5867, Adjusted R-squared: 0.555
## F-statistic: 18.46 on 1 and 13 DF, p-value: 0.0008694
## (Intercept) Time[ind]
## 0.672054090 0.009541201
## 2.5 % 97.5 %
## (Intercept) 0.429063247 0.91504493
## Time[ind] 0.004743356 0.01433905
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 2.89"
## [1] "Indices = 31" "Indices = 32" "Indices = 33" "Indices = 34"
## [5] "Indices = 35" "Indices = 36" "Indices = 37" "Indices = 38"
## [9] "Indices = 39" "Indices = 40" "Indices = 41" "Indices = 42"
## [13] "Indices = 43" "Indices = 44"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90"
## [1] "Concs = 0.504235051" "Concs = 0.597387774" "Concs = 0.684729101"
## [4] "Concs = 0.587769932" "Concs = 0.500911907" "Concs = 2.390614252"
## [7] "Concs = 0.732569141" "Concs = 0.819349521" "Concs = 0.882196657"
## [10] "Concs = 1.272480799" "Concs = 1.332879865" "Concs = 1.171215513"
## [13] "Concs = 1.284978616" "Concs = 1.466507839"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.751543 0.007487
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.36294 -0.22190 -0.11719 0.02344 1.52676
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.751543 0.197099 3.813 0.00247 **
## Time[ind] 0.007487 0.004231 1.769 0.10220
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.4801 on 12 degrees of freedom
## Multiple R-squared: 0.2069, Adjusted R-squared: 0.1408
## F-statistic: 3.131 on 1 and 12 DF, p-value: 0.1022
## (Intercept) Time[ind]
## 0.751542759 0.007487328
## 2.5 % 97.5 %
## (Intercept) 0.322101740 1.18098378
## Time[ind] -0.001732215 0.01670687
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 50"
## [1] "Indices = 45" "Indices = 46" "Indices = 47" "Indices = 48"
## [5] "Indices = 49" "Indices = 50" "Indices = 51" "Indices = 52"
## [9] "Indices = 53" "Indices = 54" "Indices = 55" "Indices = 56"
## [13] "Indices = 57" "Indices = 58" "Indices = 59"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 4.958654666" "Concs = 5.087523701" "Concs = 4.465832565"
## [4] "Concs = 2.366202494" "Concs = 2.316676352" "Concs = 2.98040696"
## [7] "Concs = 2.746818134" "Concs = 3.431666105" "Concs = 3.294563534"
## [10] "Concs = 3.820882667" "Concs = 4.627112685" "Concs = 4.219456142"
## [13] "Concs = 4.7035697" "Concs = 4.419483485" "Concs = 4.677842802"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 3.538943 0.008603
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -1.3513 -0.5950 0.1063 0.4812 1.5486
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 3.538943 0.380001 9.313 4.08e-07 ***
## Time[ind] 0.008603 0.007503 1.147 0.272
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.9389 on 13 degrees of freedom
## Multiple R-squared: 0.09183, Adjusted R-squared: 0.02198
## F-statistic: 1.315 on 1 and 13 DF, p-value: 0.2722
## (Intercept) Time[ind]
## 3.538942877 0.008602648
## 2.5 % 97.5 %
## (Intercept) 2.718000761 4.35988499
## Time[ind] -0.007606824 0.02481212
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 75"
## [1] "Indices = 60" "Indices = 61" "Indices = 62" "Indices = 63"
## [5] "Indices = 64" "Indices = 65" "Indices = 66" "Indices = 67"
## [9] "Indices = 68" "Indices = 69" "Indices = 70" "Indices = 71"
## [13] "Indices = 72" "Indices = 73" "Indices = 74"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 2.568919585" "Concs = 2.306987426" "Concs = 2.580318005"
## [4] "Concs = 2.219185884" "Concs = 2.155328801" "Concs = 2.202708256"
## [7] "Concs = 2.811019032" "Concs = 2.229132105" "Concs = 2.319209877"
## [10] "Concs = 3.438186485" "Concs = 3.239731804" "Concs = 3.112249253"
## [13] "Concs = 3.973569475" "Concs = 3.922150065" "Concs = 3.990347195"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 2.13186 0.01896
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.47150 -0.20530 0.08396 0.16046 0.44846
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 2.13186 0.11545 18.465 1.04e-10 ***
## Time[ind] 0.01896 0.00228 8.317 1.46e-06 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.2853 on 13 degrees of freedom
## Multiple R-squared: 0.8418, Adjusted R-squared: 0.8296
## F-statistic: 69.17 on 1 and 13 DF, p-value: 1.458e-06
## (Intercept) Time[ind]
## 2.13185708 0.01895929
## 2.5 % 97.5 %
## (Intercept) 1.88244004 2.38127412
## Time[ind] 0.01403456 0.02388402
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 100"
## [1] "Indices = 75" "Indices = 76" "Indices = 77" "Indices = 78"
## [5] "Indices = 79" "Indices = 80" "Indices = 81" "Indices = 82"
## [9] "Indices = 83" "Indices = 84" "Indices = 85" "Indices = 86"
## [13] "Indices = 87" "Indices = 88" "Indices = 89"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 3.426769825" "Concs = 4.801627038" "Concs = 5.167321476"
## [4] "Concs = 2.973173898" "Concs = 4.278761105" "Concs = 5.203600328"
## [7] "Concs = 3.752087918" "Concs = 4.489689601" "Concs = 4.3820044"
## [10] "Concs = 4.18858056" "Concs = 4.787062374" "Concs = 4.819935077"
## [13] "Concs = 5.229225315" "Concs = 5.541134166" "Concs = 5.363637277"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 4.11811 0.01134
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -1.31501 -0.34305 0.02152 0.31381 1.04922
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 4.118105 0.268274 15.350 1.04e-09 ***
## Time[ind] 0.011339 0.005297 2.141 0.0518 .
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.6629 on 13 degrees of freedom
## Multiple R-squared: 0.2606, Adjusted R-squared: 0.2037
## F-statistic: 4.582 on 1 and 13 DF, p-value: 0.05185
## (Intercept) Time[ind]
## 4.11810545 0.01133851
## 2.5 % 97.5 %
## (Intercept) 3.5385341200 4.69767679
## Time[ind] -0.0001051039 0.02278212
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 125"
## [1] "Indices = 90" "Indices = 91" "Indices = 92" "Indices = 93"
## [5] "Indices = 94" "Indices = 95" "Indices = 96" "Indices = 97"
## [9] "Indices = 98" "Indices = 99" "Indices = 100" "Indices = 101"
## [13] "Indices = 102" "Indices = 103" "Indices = 104"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 2.777271491" "Concs = 5.313869486" "Concs = 5.064521305"
## [4] "Concs = 3.664975002" "Concs = 5.128116042" "Concs = 3.342678541"
## [7] "Concs = 3.909877303" "Concs = 3.131834777" "Concs = 6.184369077"
## [10] "Concs = 5.034115174" "Concs = 6.997125798" "Concs = 5.513028895"
## [13] "Concs = 5.609126979" "Concs = 6.565737598" "Concs = 6.051117386"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 4.01963 0.02392
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -1.6054 -0.7704 -0.1213 0.8973 1.5423
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 4.019629 0.432639 9.291 4.19e-07 ***
## Time[ind] 0.023920 0.008542 2.800 0.015 *
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 1.069 on 13 degrees of freedom
## Multiple R-squared: 0.3762, Adjusted R-squared: 0.3282
## F-statistic: 7.841 on 1 and 13 DF, p-value: 0.01503
## (Intercept) Time[ind]
## 4.01962890 0.02392022
## 2.5 % 97.5 %
## (Intercept) 3.084968118 4.95428967
## Time[ind] 0.005465383 0.04237507
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 150"
## [1] "Indices = 105" "Indices = 106" "Indices = 107" "Indices = 108"
## [5] "Indices = 109" "Indices = 110" "Indices = 111" "Indices = 112"
## [9] "Indices = 113" "Indices = 114" "Indices = 115" "Indices = 116"
## [13] "Indices = 117" "Indices = 118" "Indices = 119"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 4.971974528" "Concs = 4.282559204" "Concs = 4.289935015"
## [4] "Concs = 4.690841414" "Concs = 4.417155339" "Concs = 6.21277087"
## [7] "Concs = 3.839544319" "Concs = 4.519625245" "Concs = 6.760429619"
## [10] "Concs = 7.2240805" "Concs = 3.468203577" "Concs = 4.462789205"
## [13] "Concs = 8.035064896" "Concs = 8.748204845" "Concs = 7.504701282"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 4.25455 0.03352
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -2.79759 -0.54038 0.03538 0.86097 1.50026
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 4.25455 0.53109 8.011 2.2e-06 ***
## Time[ind] 0.03352 0.01049 3.197 0.00701 **
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 1.312 on 13 degrees of freedom
## Multiple R-squared: 0.4401, Adjusted R-squared: 0.397
## F-statistic: 10.22 on 1 and 13 DF, p-value: 0.007014
## (Intercept) Time[ind]
## 4.25455185 0.03352069
## 2.5 % 97.5 %
## (Intercept) 3.10719564 5.40190806
## Time[ind] 0.01086618 0.05617519
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 250"
## [1] "Indices = 120" "Indices = 121" "Indices = 122" "Indices = 123"
## [5] "Indices = 124" "Indices = 125" "Indices = 126" "Indices = 127"
## [9] "Indices = 128" "Indices = 129" "Indices = 130"
## [1] "Times = 0" "Times = 0" "Times = 15" "Times = 15" "Times = 30"
## [6] "Times = 30" "Times = 60" "Times = 60" "Times = 60" "Times = 90"
## [11] "Times = 90"
## [1] "Concs = 13.83186168" "Concs = 18.22611755" "Concs = 17.81066063"
## [4] "Concs = 14.08273374" "Concs = 20.76679639" "Concs = 16.9957259"
## [7] "Concs = 18.08230553" "Concs = 16.51635254" "Concs = 17.9065353"
## [10] "Concs = 19.61630031" "Concs = 17.39520371"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 16.36742 0.02486
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -2.6576 -1.2765 0.0472 1.0407 3.6534
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 16.36742 0.98771 16.571 4.74e-08 ***
## Time[ind] 0.02486 0.01915 1.298 0.227
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 1.994 on 9 degrees of freedom
## Multiple R-squared: 0.1577, Adjusted R-squared: 0.06412
## F-statistic: 1.685 on 1 and 9 DF, p-value: 0.2265
## (Intercept) Time[ind]
## 16.3674152 0.0248645
## 2.5 % 97.5 %
## (Intercept) 14.13306576 18.60176454
## Time[ind] -0.01846508 0.06819408
## [1] "##################################################"## 'data.frame': 9 obs. of 2 variables:
## $ sub: num 0.68 10 2.89 50 75 100 125 150 250
## $ vel: num -39.3 47.7 37.4 43 94.8 ...## sub vel
## 1 0.68 -39.30888
## 2 10.00 47.70600
## 3 2.89 37.43664
## 4 50.00 43.01324
## 5 75.00 94.79647
## 6 100.00 56.69255
## 7 125.00 119.60112
## 8 150.00 167.60344
## 9 250.00 124.32252
tdlb
The above figure shows a three-dimensional plot of pesticide substrate concentration, observed concentration and experiment time. We need to condition the pesticide data on the substrate concentrations used in the experiment and regress the observed concentrations over the 90 min period in order to estimate the linear slopes for the Michaelis-Menten kinetic rate constants.
## [1] "Substrates:"## [1] "##################################################"
## [1] "Substrate = 0.68"
## [1] "Indices = 1" "Indices = 2" "Indices = 3" "Indices = 4"
## [5] "Indices = 5" "Indices = 6" "Indices = 7" "Indices = 8"
## [9] "Indices = 9" "Indices = 10" "Indices = 11" "Indices = 12"
## [13] "Indices = 13" "Indices = 14" "Indices = 15"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0.285757105" "Concs = 0.146483192" "Concs = 0.792173926"
## [4] "Concs = 0.159849035" "Concs = 0.171671782" "Concs = 0.137258352"
## [7] "Concs = 0.135498718" "Concs = 0.166579942" "Concs = 0.160555121"
## [10] "Concs = 0.085155894" "Concs = 0.137641779" "Concs = 0.101223849"
## [13] "Concs = 0.041122976" "Concs = 0.056120989" "Concs = 0.040770384"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.29736 -0.00315
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.15088 -0.07291 -0.02323 0.02705 0.49481
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.297360 0.062206 4.780 0.000359 ***
## Time[ind] -0.003150 0.001228 -2.564 0.023545 *
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.1537 on 13 degrees of freedom
## Multiple R-squared: 0.3359, Adjusted R-squared: 0.2848
## F-statistic: 6.576 on 1 and 13 DF, p-value: 0.02354
## (Intercept) Time[ind]
## 0.297360194 -0.003149641
## 2.5 % 97.5 %
## (Intercept) 0.162973379 0.4317470094
## Time[ind] -0.005803103 -0.0004961782
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 10"
## [1] "Indices = 16" "Indices = 17" "Indices = 18" "Indices = 19"
## [5] "Indices = 20" "Indices = 21" "Indices = 22" "Indices = 23"
## [9] "Indices = 24" "Indices = 25" "Indices = 26" "Indices = 27"
## [13] "Indices = 28" "Indices = 29" "Indices = 30"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0.525255948" "Concs = 0.525187009" "Concs = 0.531029336"
## [4] "Concs = 0.540578005" "Concs = 0.535327972" "Concs = 0.549947891"
## [7] "Concs = 0.963906807" "Concs = 0.939249694" "Concs = 0.8832653"
## [10] "Concs = 0.462660817" "Concs = 1.424227752" "Concs = 1.466547242"
## [13] "Concs = 1.470094516" "Concs = 1.424845158" "Concs = 1.471282702"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.49752 0.01068
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.67595 -0.07108 0.02767 0.09319 0.32794
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.497520 0.095950 5.185 0.000176 ***
## Time[ind] 0.010685 0.001895 5.640 8.07e-05 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.2371 on 13 degrees of freedom
## Multiple R-squared: 0.7099, Adjusted R-squared: 0.6876
## F-statistic: 31.81 on 1 and 13 DF, p-value: 8.067e-05
## (Intercept) Time[ind]
## 0.49752033 0.01068479
## 2.5 % 97.5 %
## (Intercept) 0.290232188 0.70480848
## Time[ind] 0.006591892 0.01477768
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 2.89"
## [1] "Indices = 31" "Indices = 32" "Indices = 33" "Indices = 34"
## [5] "Indices = 35" "Indices = 36" "Indices = 37" "Indices = 38"
## [9] "Indices = 39" "Indices = 40" "Indices = 41" "Indices = 42"
## [13] "Indices = 43" "Indices = 44" "Indices = 45"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0.200115219" "Concs = 0.242152252" "Concs = 0.309582528"
## [4] "Concs = 0.502687773" "Concs = 0.443748362" "Concs = 1.739413295"
## [7] "Concs = 0.631006803" "Concs = 0.676925019" "Concs = 0.712219102"
## [10] "Concs = 0.887492395" "Concs = 0.922424215" "Concs = 0.774293903"
## [13] "Concs = 0.733501521" "Concs = 0.837366651" "Concs = 0.76078022"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.539121 0.003909
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.33901 -0.15573 -0.05358 0.03817 1.14165
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.539121 0.145307 3.710 0.00262 **
## Time[ind] 0.003909 0.002869 1.363 0.19617
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.359 on 13 degrees of freedom
## Multiple R-squared: 0.125, Adjusted R-squared: 0.05765
## F-statistic: 1.856 on 1 and 13 DF, p-value: 0.1962
## (Intercept) Time[ind]
## 0.539120517 0.003909233
## 2.5 % 97.5 %
## (Intercept) 0.225202898 0.85303814
## Time[ind] -0.002289058 0.01010752
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 50"
## [1] "Indices = 46" "Indices = 47" "Indices = 48" "Indices = 49"
## [5] "Indices = 50" "Indices = 51" "Indices = 52" "Indices = 53"
## [9] "Indices = 54" "Indices = 55" "Indices = 56" "Indices = 57"
## [13] "Indices = 58" "Indices = 59" "Indices = 60"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 3.951483646" "Concs = 3.997004582" "Concs = 3.271884585"
## [4] "Concs = 2.598887073" "Concs = 2.355603277" "Concs = 3.141649164"
## [7] "Concs = 3.674320887" "Concs = 4.289723095" "Concs = 4.280136564"
## [10] "Concs = 6.243599629" "Concs = 6.734010485" "Concs = 6.361017452"
## [13] "Concs = 7.195467955" "Concs = 6.834134189" "Concs = 6.9532999"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 2.96383 0.04688
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -1.31143 -0.43714 -0.08051 0.52568 1.03317
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 2.963831 0.305847 9.691 2.59e-07 ***
## Time[ind] 0.046880 0.006039 7.763 3.10e-06 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.7557 on 13 degrees of freedom
## Multiple R-squared: 0.8226, Adjusted R-squared: 0.8089
## F-statistic: 60.26 on 1 and 13 DF, p-value: 3.103e-06
## (Intercept) Time[ind]
## 2.96383098 0.04687993
## 2.5 % 97.5 %
## (Intercept) 2.30308946 3.62457250
## Time[ind] 0.03383361 0.05992624
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 75"
## [1] "Indices = 61" "Indices = 62" "Indices = 63" "Indices = 64"
## [5] "Indices = 65" "Indices = 66" "Indices = 67" "Indices = 68"
## [9] "Indices = 69" "Indices = 70" "Indices = 71" "Indices = 72"
## [13] "Indices = 73" "Indices = 74" "Indices = 75"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 1.941666948" "Concs = 1.681717791" "Concs = 1.73936846"
## [4] "Concs = 2.526757358" "Concs = 2.313637237" "Concs = 2.49086012"
## [7] "Concs = 3.789628052" "Concs = 3.174642429" "Concs = 3.191438558"
## [10] "Concs = 5.432233942" "Concs = 4.969157891" "Concs = 5.064503301"
## [13] "Concs = 6.712664257" "Concs = 6.597464296" "Concs = 6.484914"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 1.73768 0.05478
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.24572 -0.12656 -0.05524 0.04251 0.40859
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 1.73768 0.08458 20.55 2.7e-11 ***
## Time[ind] 0.05478 0.00167 32.80 6.9e-14 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.209 on 13 degrees of freedom
## Multiple R-squared: 0.9881, Adjusted R-squared: 0.9871
## F-statistic: 1076 on 1 and 13 DF, p-value: 6.901e-14
## (Intercept) Time[ind]
## 1.73768018 0.05477855
## 2.5 % 97.5 %
## (Intercept) 1.55496133 1.92039902
## Time[ind] 0.05117077 0.05838633
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 100"
## [1] "Indices = 76" "Indices = 77" "Indices = 78" "Indices = 79"
## [5] "Indices = 80" "Indices = 81" "Indices = 82" "Indices = 83"
## [9] "Indices = 84" "Indices = 85" "Indices = 86" "Indices = 87"
## [13] "Indices = 88" "Indices = 89" "Indices = 90"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 2.204827173" "Concs = 3.695255037" "Concs = 4.217693628"
## [4] "Concs = 2.987986855" "Concs = 4.62322369" "Concs = 5.738654903"
## [7] "Concs = 5.010985215" "Concs = 6.920302664" "Concs = 6.308852644"
## [10] "Concs = 8.11715413" "Concs = 8.804687457" "Concs = 8.950282552"
## [13] "Concs = 10.81651263" "Concs = 11.45788264" "Concs = 11.01782631"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 3.33269 0.08698
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -1.6494 -0.3892 0.2534 0.3829 1.1013
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 3.332689 0.331419 10.06 1.69e-07 ***
## Time[ind] 0.086977 0.006544 13.29 6.08e-09 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.8189 on 13 degrees of freedom
## Multiple R-squared: 0.9315, Adjusted R-squared: 0.9262
## F-statistic: 176.7 on 1 and 13 DF, p-value: 6.077e-09
## (Intercept) Time[ind]
## 3.33268858 0.08697743
## 2.5 % 97.5 %
## (Intercept) 2.6167016 4.0486755
## Time[ind] 0.0728403 0.1011146
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 125"
## [1] "Indices = 91" "Indices = 92" "Indices = 93" "Indices = 94"
## [5] "Indices = 95" "Indices = 96" "Indices = 97" "Indices = 98"
## [9] "Indices = 99" "Indices = 100" "Indices = 101" "Indices = 102"
## [13] "Indices = 103" "Indices = 104" "Indices = 105"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 2.580796931" "Concs = 4.589962599" "Concs = 4.339108289"
## [4] "Concs = 4.759629498" "Concs = 6.081131385" "Concs = 4.761075887"
## [7] "Concs = 6.970865526" "Concs = 5.864292522" "Concs = 8.990170569"
## [10] "Concs = 10.29254833" "Concs = 11.89986315" "Concs = 10.55439287"
## [13] "Concs = 12.66145718" "Concs = 13.84087365" "Concs = 12.77355717"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 3.9025 0.1067
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -1.32170 -0.74272 -0.01223 0.50734 1.88653
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 3.902497 0.403600 9.669 2.65e-07 ***
## Time[ind] 0.106705 0.007969 13.390 5.55e-09 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.9972 on 13 degrees of freedom
## Multiple R-squared: 0.9324, Adjusted R-squared: 0.9272
## F-statistic: 179.3 on 1 and 13 DF, p-value: 5.555e-09
## (Intercept) Time[ind]
## 3.9024967 0.1067047
## 2.5 % 97.5 %
## (Intercept) 3.03057157 4.7744218
## Time[ind] 0.08948862 0.1239209
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 150"
## [1] "Indices = 106" "Indices = 107" "Indices = 108" "Indices = 109"
## [5] "Indices = 110" "Indices = 111" "Indices = 112" "Indices = 113"
## [9] "Indices = 114" "Indices = 115" "Indices = 116" "Indices = 117"
## [13] "Indices = 118" "Indices = 119" "Indices = 120"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 4.642200138" "Concs = 4.107381982" "Concs = 3.642209544"
## [4] "Concs = 6.090475637" "Concs = 5.80525893" "Concs = 8.98551562"
## [7] "Concs = 6.941039356" "Concs = 7.206821805" "Concs = 10.94796175"
## [10] "Concs = 13.80091931" "Concs = 7.457397291" "Concs = 9.668427529"
## [13] "Concs = 16.81467648" "Concs = 18.96446187" "Concs = 16.539466"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 4.1926 0.1346
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -4.8096 -0.7867 -0.0852 1.0222 2.7743
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 4.1926 0.8557 4.900 0.00029 ***
## Time[ind] 0.1346 0.0169 7.965 2.35e-06 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 2.114 on 13 degrees of freedom
## Multiple R-squared: 0.8299, Adjusted R-squared: 0.8169
## F-statistic: 63.44 on 1 and 13 DF, p-value: 2.346e-06
## (Intercept) Time[ind]
## 4.1925881 0.1345733
## 2.5 % 97.5 %
## (Intercept) 2.34396600 6.0412103
## Time[ind] 0.09807234 0.1710743
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 250"
## [1] "Indices = 121" "Indices = 122" "Indices = 123" "Indices = 124"
## [5] "Indices = 125" "Indices = 126" "Indices = 127" "Indices = 128"
## [9] "Indices = 129" "Indices = 130" "Indices = 131"
## [1] "Times = 0" "Times = 0" "Times = 15" "Times = 15" "Times = 30"
## [6] "Times = 30" "Times = 60" "Times = 60" "Times = 60" "Times = 90"
## [11] "Times = 90"
## [1] "Concs = 19.16500098" "Concs = 25.91267967" "Concs = 22.19280552"
## [4] "Concs = 17.62020275" "Concs = 24.15778887" "Concs = 20.49970666"
## [7] "Concs = 25.33183553" "Concs = 23.63873667" "Concs = 25.85088774"
## [10] "Concs = 28.34728168" "Concs = 25.77673742"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 20.77252 0.06666
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -4.1522 -1.3704 0.4204 1.2322 5.1402
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 20.77252 1.27599 16.279 5.53e-08 ***
## Time[ind] 0.06666 0.02474 2.694 0.0246 *
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 2.576 on 9 degrees of freedom
## Multiple R-squared: 0.4464, Adjusted R-squared: 0.3849
## F-statistic: 7.257 on 1 and 9 DF, p-value: 0.02464
## (Intercept) Time[ind]
## 20.77252385 0.06665756
## 2.5 % 97.5 %
## (Intercept) 17.88602380 23.659024
## Time[ind] 0.01068116 0.122634
## [1] "##################################################"## 'data.frame': 9 obs. of 2 variables:
## $ sub: num 0.68 10 2.89 50 75 100 125 150 250
## $ vel: num -15.7 53.4 19.5 234.4 273.9 ...## sub vel
## 1 0.68 -15.74820
## 2 10.00 53.42394
## 3 2.89 19.54617
## 4 50.00 234.39964
## 5 75.00 273.89275
## 6 100.00 434.88717
## 7 125.00 533.52372
## 8 150.00 672.86659
## 9 250.00 333.28779
fipronil
The above figure shows a three-dimensional plot of pesticide substrate concentration, observed concentration and experiment time. We need to condition the pesticide data on the substrate concentrations used in the experiment and regress the observed concentrations over the 90 min period in order to estimate the linear slopes for the Michaelis-Menten kinetic rate constants.
## [1] "Substrates:"## [1] "##################################################"
## [1] "Substrate = 10"
## [1] "Indices = 1" "Indices = 2" "Indices = 3" "Indices = 4"
## [5] "Indices = 5" "Indices = 6" "Indices = 7" "Indices = 8"
## [9] "Indices = 9" "Indices = 10" "Indices = 11" "Indices = 12"
## [13] "Indices = 13" "Indices = 14"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 30" "Times = 30" "Times = 30" "Times = 60" "Times = 60"
## [11] "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 105.7355056" "Concs = 71.31297707" "Concs = 79.81665852"
## [4] "Concs = 7.601993848" "Concs = 11.87171779" "Concs = 8.347482218"
## [7] "Concs = 12.54233702" "Concs = 9.213313367" "Concs = 10.38856477"
## [10] "Concs = 12.36949156" "Concs = 8.215365646" "Concs = 11.17931895"
## [13] "Concs = 12.71923746" "Concs = 9.99477721"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 50.9177 -0.5992
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -34.328 -22.896 -3.587 15.344 54.818
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 50.9177 11.4177 4.460 0.00078 ***
## Time[ind] -0.5992 0.2184 -2.743 0.01783 *
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 26.79 on 12 degrees of freedom
## Multiple R-squared: 0.3854, Adjusted R-squared: 0.3342
## F-statistic: 7.525 on 1 and 12 DF, p-value: 0.01783
## (Intercept) Time[ind]
## 50.9177421 -0.5991924
## 2.5 % 97.5 %
## (Intercept) 26.040763 75.7947212
## Time[ind] -1.075125 -0.1232592
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 2.89"
## [1] "Indices = 15" "Indices = 16" "Indices = 17" "Indices = 18"
## [5] "Indices = 19" "Indices = 20" "Indices = 21" "Indices = 22"
## [9] "Indices = 23" "Indices = 24" "Indices = 25" "Indices = 26"
## [13] "Indices = 27" "Indices = 28" "Indices = 29"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 3.553166435" "Concs = 2.185797914" "Concs = 4.160901096"
## [4] "Concs = 0.961731316" "Concs = 0.608701865" "Concs = 2.021395291"
## [7] "Concs = 0.34095865" "Concs = 1.416552854" "Concs = 4.678731136"
## [10] "Concs = 1.597568113" "Concs = 2.059112975" "Concs = 4.336224939"
## [13] "Concs = 0.73593197" "Concs = 1.01143428" "Concs = 2.098949857"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 2.53175 -0.01061
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -1.8724 -0.8187 -0.3459 0.7719 2.4654
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 2.53175 0.57930 4.370 0.000758 ***
## Time[ind] -0.01061 0.01144 -0.928 0.370357
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 1.431 on 13 degrees of freedom
## Multiple R-squared: 0.06212, Adjusted R-squared: -0.01003
## F-statistic: 0.861 on 1 and 13 DF, p-value: 0.3704
## (Intercept) Time[ind]
## 2.53174769 -0.01061377
## 2.5 % 97.5 %
## (Intercept) 1.28025077 3.78324461
## Time[ind] -0.03532453 0.01409699
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 50"
## [1] "Indices = 30" "Indices = 31" "Indices = 32" "Indices = 33"
## [5] "Indices = 34" "Indices = 35" "Indices = 36" "Indices = 37"
## [9] "Indices = 38" "Indices = 39" "Indices = 40" "Indices = 41"
## [13] "Indices = 42" "Indices = 43" "Indices = 44"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 285.0383486" "Concs = 295.6872209" "Concs = 261.2375276"
## [4] "Concs = 223.3849191" "Concs = 19.09530124" "Concs = 61.85004879"
## [7] "Concs = 76.72890033" "Concs = 65.4447593" "Concs = 59.88633813"
## [10] "Concs = 74.09066447" "Concs = 65.54242812" "Concs = 39.55208359"
## [13] "Concs = 66.15498564" "Concs = 23.55175796" "Concs = 58.25965938"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 188.031 -1.957
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -139.578 -58.228 3.492 59.492 107.656
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 188.0314 31.2086 6.025 4.27e-05 ***
## Time[ind] -1.9572 0.6162 -3.176 0.00729 **
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 77.11 on 13 degrees of freedom
## Multiple R-squared: 0.4369, Adjusted R-squared: 0.3936
## F-statistic: 10.09 on 1 and 13 DF, p-value: 0.007294
## (Intercept) Time[ind]
## 188.031379 -1.957206
## 2.5 % 97.5 %
## (Intercept) 120.609359 255.4533982
## Time[ind] -3.288452 -0.6259611
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 75"
## [1] "Indices = 45" "Indices = 46" "Indices = 47" "Indices = 48"
## [5] "Indices = 49" "Indices = 50" "Indices = 51" "Indices = 52"
## [9] "Indices = 53" "Indices = 54" "Indices = 55" "Indices = 56"
## [13] "Indices = 57" "Indices = 58"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 49.56034014" "Concs = 49.17468426" "Concs = 36.68866915"
## [4] "Concs = 35.05455395" "Concs = 40.30349338" "Concs = 44.1381674"
## [7] "Concs = 38.08049817" "Concs = 38.34527612" "Concs = 89.31881211"
## [10] "Concs = 40.27279664" "Concs = 55.95173867" "Concs = 37.82415225"
## [13] "Concs = 32.32010713" "Concs = 45.93427684"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 47.06620 -0.04945
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -11.270 -7.436 -4.309 2.398 43.736
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 47.06620 5.96482 7.891 4.33e-06 ***
## Time[ind] -0.04945 0.11951 -0.414 0.686
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 14.73 on 12 degrees of freedom
## Multiple R-squared: 0.01406, Adjusted R-squared: -0.0681
## F-statistic: 0.1712 on 1 and 12 DF, p-value: 0.6864
## (Intercept) Time[ind]
## 47.06619751 -0.04944609
## 2.5 % 97.5 %
## (Intercept) 34.0699795 60.0624155
## Time[ind] -0.3098359 0.2109437
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 100"
## [1] "Indices = 59" "Indices = 60" "Indices = 61" "Indices = 62"
## [5] "Indices = 63" "Indices = 64" "Indices = 65" "Indices = 66"
## [9] "Indices = 67" "Indices = 68" "Indices = 69" "Indices = 70"
## [13] "Indices = 71" "Indices = 72" "Indices = 73"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 32.55124669" "Concs = 72.28565415" "Concs = 50.30200713"
## [4] "Concs = 65.75118371" "Concs = 61.03836189" "Concs = 86.42270417"
## [7] "Concs = 18.77293974" "Concs = 84.04356305" "Concs = 89.30314297"
## [10] "Concs = 60.95565287" "Concs = 89.79580177" "Concs = 90.59062961"
## [13] "Concs = 56.03613412" "Concs = 94.58659729" "Concs = 89.70085579"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 58.4479 0.2828
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -48.158 -11.302 5.804 14.779 23.733
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 58.4479 8.8289 6.620 1.66e-05 ***
## Time[ind] 0.2828 0.1743 1.622 0.129
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 21.82 on 13 degrees of freedom
## Multiple R-squared: 0.1683, Adjusted R-squared: 0.1043
## F-statistic: 2.631 on 1 and 13 DF, p-value: 0.1288
## (Intercept) Time[ind]
## 58.4479335 0.2827649
## 2.5 % 97.5 %
## (Intercept) 39.37416273 77.5217042
## Time[ind] -0.09384598 0.6593758
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 125"
## [1] "Indices = 74" "Indices = 75" "Indices = 76" "Indices = 77"
## [5] "Indices = 78" "Indices = 79" "Indices = 80" "Indices = 81"
## [9] "Indices = 82" "Indices = 83" "Indices = 84" "Indices = 85"
## [13] "Indices = 86" "Indices = 87" "Indices = 88"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 58.45901219" "Concs = 51.85481179" "Concs = 53.89065136"
## [4] "Concs = 62.24891641" "Concs = 54.68420677" "Concs = 91.10795719"
## [7] "Concs = 51.66702524" "Concs = 65.6950903" "Concs = 112.1237445"
## [10] "Concs = 62.81350539" "Concs = 74.14828799" "Concs = 93.87631612"
## [13] "Concs = 59.80299767" "Concs = 82.33654045" "Concs = 89.20514072"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 62.9985 0.2033
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -21.493 -11.254 -3.799 4.474 43.026
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 62.9985 7.2701 8.665 9.23e-07 ***
## Time[ind] 0.2033 0.1435 1.416 0.18
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 17.96 on 13 degrees of freedom
## Multiple R-squared: 0.1337, Adjusted R-squared: 0.06704
## F-statistic: 2.006 on 1 and 13 DF, p-value: 0.1802
## (Intercept) Time[ind]
## 62.9984959 0.2033107
## 2.5 % 97.5 %
## (Intercept) 47.2924980 78.7044939
## Time[ind] -0.1068036 0.5134251
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 150"
## [1] "Indices = 89" "Indices = 90" "Indices = 91" "Indices = 92"
## [5] "Indices = 93" "Indices = 94" "Indices = 95" "Indices = 96"
## [9] "Indices = 97" "Indices = 98" "Indices = 99" "Indices = 100"
## [13] "Indices = 101" "Indices = 102" "Indices = 103"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 80.1226913" "Concs = 96.90138648" "Concs = 94.88883577"
## [4] "Concs = 37.15191594" "Concs = 76.95257401" "Concs = 82.83018255"
## [7] "Concs = 89.28298886" "Concs = 103.9076465" "Concs = 88.89209516"
## [10] "Concs = 75.20034761" "Concs = 99.21349783" "Concs = 86.10641447"
## [13] "Concs = 85.35534061" "Concs = 86.89358929" "Concs = 19.45762438"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 86.7399 -0.1674
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -52.214 -4.056 7.566 11.922 22.519
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 86.7399 9.2758 9.351 3.9e-07 ***
## Time[ind] -0.1674 0.1832 -0.914 0.377
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 22.92 on 13 degrees of freedom
## Multiple R-squared: 0.0604, Adjusted R-squared: -0.01188
## F-statistic: 0.8356 on 1 and 13 DF, p-value: 0.3773
## (Intercept) Time[ind]
## 86.7399137 -0.1674215
## 2.5 % 97.5 %
## (Intercept) 66.7007990 106.7790284
## Time[ind] -0.5630931 0.2282501
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 200"
## [1] "Indices = 104" "Indices = 105" "Indices = 106" "Indices = 107"
## [5] "Indices = 108" "Indices = 109" "Indices = 110" "Indices = 111"
## [9] "Indices = 112" "Indices = 113" "Indices = 114" "Indices = 115"
## [13] "Indices = 116" "Indices = 117" "Indices = 118"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 161.3373216" "Concs = 140.3753919" "Concs = 143.641415"
## [4] "Concs = 143.5820327" "Concs = 117.0381728" "Concs = 105.3992543"
## [7] "Concs = 120.601107" "Concs = 115.1379412" "Concs = 126.1830373"
## [10] "Concs = 91.91948648" "Concs = 106.6462813" "Concs = 72.50149496"
## [13] "Concs = 112.4063583" "Concs = 105.3992543" "Concs = 111.7531537"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 135.4719 -0.4413
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -36.493 -9.455 3.950 12.187 25.865
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 135.4719 7.0902 19.107 6.75e-11 ***
## Time[ind] -0.4413 0.1400 -3.152 0.00764 **
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 17.52 on 13 degrees of freedom
## Multiple R-squared: 0.4332, Adjusted R-squared: 0.3896
## F-statistic: 9.936 on 1 and 13 DF, p-value: 0.00764
## (Intercept) Time[ind]
## 135.4719208 -0.4412942
## 2.5 % 97.5 %
## (Intercept) 120.1543894 150.7894522
## Time[ind] -0.7437383 -0.1388501
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 250"
## [1] "Indices = 119" "Indices = 120" "Indices = 121" "Indices = 122"
## [5] "Indices = 123" "Indices = 124" "Indices = 125" "Indices = 126"
## [9] "Indices = 127" "Indices = 128" "Indices = 129" "Indices = 130"
## [13] "Indices = 131" "Indices = 132" "Indices = 133"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 94.37993663" "Concs = 113.4786371" "Concs = 91.29136792"
## [4] "Concs = 105.2844752" "Concs = 83.9166222" "Concs = 57.25407999"
## [7] "Concs = 71.31021926" "Concs = 93.93871253" "Concs = 36.95777127"
## [10] "Concs = 77.4873567" "Concs = 101.3134582" "Concs = 91.54349597"
## [13] "Concs = 58.38865626" "Concs = 74.14665992" "Concs = 61.1620649"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 90.3680 -0.2456
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -46.043 -8.490 1.854 13.424 25.680
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 90.3680 8.1298 11.12 5.2e-08 ***
## Time[ind] -0.2456 0.1605 -1.53 0.15
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 20.09 on 13 degrees of freedom
## Multiple R-squared: 0.1526, Adjusted R-squared: 0.08739
## F-statistic: 2.341 on 1 and 13 DF, p-value: 0.15
## (Intercept) Time[ind]
## 90.3679851 -0.2455834
## 2.5 % 97.5 %
## (Intercept) 72.8046674 107.9313029
## Time[ind] -0.5923704 0.1012037
## [1] "##################################################"## 'data.frame': 9 obs. of 2 variables:
## $ sub: num 10 2.89 50 75 100 125 150 200 250
## $ vel: num 2996 53.1 9786 247.2 -1413.8 ...## sub vel
## 1 10.00 2995.96183
## 2 2.89 53.06886
## 3 50.00 9786.03192
## 4 75.00 247.23047
## 5 100.00 -1413.82455
## 6 125.00 -1016.55355
## 7 150.00 837.10748
## 8 200.00 2206.47101
## 9 250.00 1227.91678
fipsulf
The above figure shows a three-dimensional plot of pesticide substrate concentration, observed concentration and experiment time. We need to condition the pesticide data on the substrate concentrations used in the experiment and regress the observed concentrations over the 90 min period in order to estimate the linear slopes for the Michaelis-Menten kinetic rate constants.
## [1] "Substrates:"## [1] "##################################################"
## [1] "Substrate = 10"
## [1] "Indices = 1" "Indices = 2" "Indices = 3" "Indices = 4"
## [5] "Indices = 5" "Indices = 6" "Indices = 7" "Indices = 8"
## [9] "Indices = 9" "Indices = 10" "Indices = 11" "Indices = 12"
## [13] "Indices = 13" "Indices = 14" "Indices = 15"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0.125996797" "Concs = 0.047756822" "Concs = 0.057531836"
## [4] "Concs = 0.226052779" "Concs = 0.249546426" "Concs = 0.249495111"
## [7] "Concs = 0.556306401" "Concs = 0.629523416" "Concs = 0.545734442"
## [10] "Concs = 1.029532274" "Concs = 1.020828782" "Concs = 1.121529265"
## [13] "Concs = 1.478049545" "Concs = 1.552223152" "Concs = 1.605171177"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.04966 0.01667
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.073616 -0.039497 -0.001901 0.031662 0.079844
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.0496582 0.0217059 2.288 0.0395 *
## Time[ind] 0.0166674 0.0004286 38.889 7.71e-15 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.05363 on 13 degrees of freedom
## Multiple R-squared: 0.9915, Adjusted R-squared: 0.9908
## F-statistic: 1512 on 1 and 13 DF, p-value: 7.707e-15
## (Intercept) Time[ind]
## 0.04965817 0.01666736
## 2.5 % 97.5 %
## (Intercept) 0.002765368 0.09655097
## Time[ind] 0.015741464 0.01759326
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 2.89"
## [1] "Indices = 16" "Indices = 17" "Indices = 18" "Indices = 19"
## [5] "Indices = 20" "Indices = 21" "Indices = 22" "Indices = 23"
## [9] "Indices = 24" "Indices = 25" "Indices = 26" "Indices = 27"
## [13] "Indices = 28" "Indices = 29" "Indices = 30"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0" "Concs = 0" "Concs = 0"
## [7] "Concs = 0.072417652" "Concs = 0.105189206" "Concs = 0.093830232"
## [10] "Concs = 0.446059868" "Concs = 0.501873862" "Concs = 1.937676963"
## [13] "Concs = 0.541481009" "Concs = 0.713021528" "Concs = 0.948269563"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## -0.06729 0.01089
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.37110 -0.15983 -0.09602 0.05149 1.35172
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) -0.067286 0.164967 -0.408 0.6900
## Time[ind] 0.010887 0.003257 3.342 0.0053 **
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.4076 on 13 degrees of freedom
## Multiple R-squared: 0.4622, Adjusted R-squared: 0.4208
## F-statistic: 11.17 on 1 and 13 DF, p-value: 0.005297
## (Intercept) Time[ind]
## -0.06728624 0.01088737
## 2.5 % 97.5 %
## (Intercept) -0.423675199 0.28910272
## Time[ind] 0.003850487 0.01792426
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 50"
## [1] "Indices = 31" "Indices = 32" "Indices = 33" "Indices = 34"
## [5] "Indices = 35" "Indices = 36" "Indices = 37" "Indices = 38"
## [9] "Indices = 39" "Indices = 40" "Indices = 41" "Indices = 42"
## [13] "Indices = 43" "Indices = 44" "Indices = 45"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 1.232923543" "Concs = 6.086978824" "Concs = 0.564979782"
## [4] "Concs = 2.509901494" "Concs = 0.497072024" "Concs = 0.573048602"
## [7] "Concs = 0.208702278" "Concs = 1.224307072" "Concs = 0.86186791"
## [10] "Concs = 2.640613398" "Concs = 2.278728642" "Concs = 1.938091721"
## [13] "Concs = 3.288556396" "Concs = 2.79424236" "Concs = 2.85277407"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 1.50788 0.01185
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -1.6548 -0.9723 -0.2750 0.3498 4.5791
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 1.50788 0.61796 2.440 0.0298 *
## Time[ind] 0.01185 0.01220 0.972 0.3490
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 1.527 on 13 degrees of freedom
## Multiple R-squared: 0.06769, Adjusted R-squared: -0.004025
## F-statistic: 0.9439 on 1 and 13 DF, p-value: 0.349
## (Intercept) Time[ind]
## 1.50787526 0.01185412
## 2.5 % 97.5 %
## (Intercept) 0.17286420 2.84288632
## Time[ind] -0.01450563 0.03821386
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 75"
## [1] "Indices = 46" "Indices = 47" "Indices = 48" "Indices = 49"
## [5] "Indices = 50" "Indices = 51" "Indices = 52" "Indices = 53"
## [9] "Indices = 54" "Indices = 55" "Indices = 56" "Indices = 57"
## [13] "Indices = 58" "Indices = 59"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.61596018" "Concs = 0.004496637" "Concs = 0.393574273"
## [7] "Concs = 1.00896852" "Concs = 1.080335369" "Concs = 1.122428808"
## [10] "Concs = 0.794034486" "Concs = 1.567175192" "Concs = 1.445091993"
## [13] "Concs = 1.567376573" "Concs = 1.397216044"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.19848 0.01565
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.4288 -0.1985 -0.1010 0.3013 0.4543
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.19848 0.12829 1.547 0.148
## Time[ind] 0.01565 0.00257 6.090 5.42e-05 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.3168 on 12 degrees of freedom
## Multiple R-squared: 0.7555, Adjusted R-squared: 0.7351
## F-statistic: 37.08 on 1 and 12 DF, p-value: 5.421e-05
## (Intercept) Time[ind]
## 0.19848307 0.01565313
## 2.5 % 97.5 %
## (Intercept) -0.08104254 0.47800868
## Time[ind] 0.01005261 0.02125366
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 100"
## [1] "Indices = 60" "Indices = 61" "Indices = 62" "Indices = 63"
## [5] "Indices = 64" "Indices = 65" "Indices = 66" "Indices = 67"
## [9] "Indices = 68" "Indices = 69" "Indices = 70" "Indices = 71"
## [13] "Indices = 72" "Indices = 73" "Indices = 74"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0.064056262" "Concs = 0.084903651" "Concs = 0.054098343"
## [4] "Concs = 1.037370756" "Concs = 1.004548869" "Concs = 1.211451943"
## [7] "Concs = 1.770038982" "Concs = 1.964421756" "Concs = 2.082784287"
## [10] "Concs = 2.421230649" "Concs = 2.716290502" "Concs = 2.511880669"
## [13] "Concs = 2.884476336" "Concs = 3.658963096" "Concs = 3.101044324"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.49072 0.03283
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.56113 -0.37519 0.05124 0.24195 0.60710
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.490719 0.152077 3.227 0.00662 **
## Time[ind] 0.032832 0.003003 10.934 6.32e-08 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.3758 on 13 degrees of freedom
## Multiple R-squared: 0.9019, Adjusted R-squared: 0.8944
## F-statistic: 119.6 on 1 and 13 DF, p-value: 6.325e-08
## (Intercept) Time[ind]
## 0.4907187 0.0328321
## 2.5 % 97.5 %
## (Intercept) 0.16217637 0.81926107
## Time[ind] 0.02634505 0.03931916
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 125"
## [1] "Indices = 75" "Indices = 76" "Indices = 77" "Indices = 78"
## [5] "Indices = 79" "Indices = 80" "Indices = 81" "Indices = 82"
## [9] "Indices = 83" "Indices = 84" "Indices = 85" "Indices = 86"
## [13] "Indices = 87" "Indices = 88" "Indices = 89"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 1.0685532" "Concs = 1.193964217" "Concs = 1.061157254"
## [7] "Concs = 1.739690158" "Concs = 1.568026457" "Concs = 1.831902381"
## [10] "Concs = 2.58185903" "Concs = 2.518118409" "Concs = 2.706182414"
## [13] "Concs = 3.388713261" "Concs = 3.265199632" "Concs = 3.360717697"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.38590 0.03504
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.38590 -0.22611 0.09385 0.18765 0.39495
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.385901 0.113655 3.395 0.00478 **
## Time[ind] 0.035035 0.002244 15.612 8.42e-10 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.2808 on 13 degrees of freedom
## Multiple R-squared: 0.9494, Adjusted R-squared: 0.9455
## F-statistic: 243.7 on 1 and 13 DF, p-value: 8.42e-10
## (Intercept) Time[ind]
## 0.38590066 0.03503517
## 2.5 % 97.5 %
## (Intercept) 0.14036476 0.63143656
## Time[ind] 0.03018707 0.03988327
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 150"
## [1] "Indices = 90" "Indices = 91" "Indices = 92" "Indices = 93"
## [5] "Indices = 94" "Indices = 95" "Indices = 96" "Indices = 97"
## [9] "Indices = 98" "Indices = 99" "Indices = 100" "Indices = 101"
## [13] "Indices = 102" "Indices = 103" "Indices = 104"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0.319307475" "Concs = 0.322811871" "Concs = 0.334641014"
## [4] "Concs = 1.513101462" "Concs = 1.254618226" "Concs = 1.316915121"
## [7] "Concs = 1.847166036" "Concs = 2.06265471" "Concs = 2.225655093"
## [10] "Concs = 3.029089226" "Concs = 2.818619179" "Concs = 2.892261352"
## [13] "Concs = 3.395108251" "Concs = 3.493680617" "Concs = 3.346621743"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.73091 0.03284
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.41160 -0.31522 0.09348 0.24045 0.50969
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.730906 0.131800 5.546 9.46e-05 ***
## Time[ind] 0.032835 0.002602 12.617 1.14e-08 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.3257 on 13 degrees of freedom
## Multiple R-squared: 0.9245, Adjusted R-squared: 0.9187
## F-statistic: 159.2 on 1 and 13 DF, p-value: 1.142e-08
## (Intercept) Time[ind]
## 0.73090589 0.03283532
## 2.5 % 97.5 %
## (Intercept) 0.44616859 1.01564319
## Time[ind] 0.02721319 0.03845745
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 200"
## [1] "Indices = 105" "Indices = 106" "Indices = 107" "Indices = 108"
## [5] "Indices = 109" "Indices = 110" "Indices = 111" "Indices = 112"
## [9] "Indices = 113" "Indices = 114" "Indices = 115" "Indices = 116"
## [13] "Indices = 117" "Indices = 118" "Indices = 119"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0.139500206" "Concs = 0"
## [4] "Concs = 0.921840151" "Concs = 1.265455207" "Concs = 0.083794693"
## [7] "Concs = 1.758009212" "Concs = 1.131761977" "Concs = 2.14032494"
## [10] "Concs = 3.119569212" "Concs = 5.219960219" "Concs = 3.68483357"
## [13] "Concs = 4.382618411" "Concs = 4.121095689" "Concs = 4.42249183"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.1508 0.0515
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.8396 -0.3835 -0.1214 0.2021 1.9790
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.150816 0.280672 0.537 0.6
## Time[ind] 0.051503 0.005542 9.293 4.18e-07 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.6935 on 13 degrees of freedom
## Multiple R-squared: 0.8692, Adjusted R-squared: 0.8591
## F-statistic: 86.37 on 1 and 13 DF, p-value: 4.182e-07
## (Intercept) Time[ind]
## 0.15081593 0.05150259
## 2.5 % 97.5 %
## (Intercept) -0.45553864 0.75717051
## Time[ind] 0.03953014 0.06347504
## [1] "##################################################"
## [1] "##################################################"
## [1] "Substrate = 250"
## [1] "Indices = 120" "Indices = 121" "Indices = 122" "Indices = 123"
## [5] "Indices = 124" "Indices = 125" "Indices = 126" "Indices = 127"
## [9] "Indices = 128" "Indices = 129" "Indices = 130" "Indices = 131"
## [13] "Indices = 132" "Indices = 133" "Indices = 134"
## [1] "Times = 0" "Times = 0" "Times = 0" "Times = 15" "Times = 15"
## [6] "Times = 15" "Times = 30" "Times = 30" "Times = 30" "Times = 60"
## [11] "Times = 60" "Times = 60" "Times = 90" "Times = 90" "Times = 90"
## [1] "Concs = 0" "Concs = 0" "Concs = 0"
## [4] "Concs = 0.688691412" "Concs = 0.680203691" "Concs = 0.671715969"
## [7] "Concs = 1.128032043" "Concs = 1.057705208" "Concs = 1.0791266"
## [10] "Concs = 1.670842039" "Concs = 1.655483305" "Concs = 1.672054571"
## [13] "Concs = 2.11584115" "Concs = 2.394723427" "Concs = 2.276703681"
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Coefficients:
## (Intercept) Time[ind]
## 0.2111 0.0238
##
##
## Call:
## lm(formula = Observed[ind] ~ Time[ind])
##
## Residuals:
## Min 1Q Median 3Q Max
## -0.23753 -0.14388 0.03278 0.11632 0.20285
##
## Coefficients:
## Estimate Std. Error t value Pr(>|t|)
## (Intercept) 0.211083 0.063521 3.323 0.0055 **
## Time[ind] 0.023803 0.001254 18.978 7.35e-11 ***
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Residual standard error: 0.157 on 13 degrees of freedom
## Multiple R-squared: 0.9652, Adjusted R-squared: 0.9625
## F-statistic: 360.2 on 1 and 13 DF, p-value: 7.353e-11
## (Intercept) Time[ind]
## 0.21108314 0.02380321
## 2.5 % 97.5 %
## (Intercept) 0.07385384 0.34831244
## Time[ind] 0.02109362 0.02651279
## [1] "##################################################"## 'data.frame': 9 obs. of 2 variables:
## $ sub: num 10 2.89 50 75 100 125 150 200 250
## $ vel: num 83.3 54.4 59.3 78.3 164.2 ...## sub vel
## 1 10.00 83.33680
## 2 2.89 54.43687
## 3 50.00 59.27059
## 4 75.00 78.26567
## 5 100.00 164.16051
## 6 125.00 175.17585
## 7 150.00 164.17661
## 8 200.00 257.51296
## 9 250.00 119.01603
exposure experiment analysis
Results presentation and discussion for parent analytes (atrazine, triadimenon, and fipronil) and their xenobiotic metabolites.
Toxicity of parents and metabolites discussion.
Microsomal analysis of soil and amphibian data for 0 (soil only), 2, 4, 12, 24, and 48 hours after exposure.
Database has factors for time, parent (mapped to analyte), analyte (can be either parent or metabolite), matrix (amphibian or soil), and tank (potentially a nuisance variable).
## Classes 'grouped_df', 'tbl_df', 'tbl' and 'data.frame': 352 obs. of 6 variables:
## $ time : int 2 2 2 2 4 4 4 4 12 12 ...
## $ parent : Factor w/ 3 levels "atrazine","fipronil",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ analyte : Factor w/ 8 levels "atrazine","dea",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ matrix : Factor w/ 2 levels "amphib","soil": 1 1 1 1 1 1 1 1 1 1 ...
## $ conc : num 3.07 3.69 4.7 1.89 7.62 ...
## $ replicate: Factor w/ 4 levels "1","2","3","4": 1 2 3 4 1 2 3 4 1 2 ...
## - attr(*, "vars")=List of 4
## ..$ : symbol parent
## ..$ : symbol analyte
## ..$ : symbol matrix
## ..$ : symbol time
## - attr(*, "drop")= logi TRUE
## - attr(*, "indices")=List of 86
## ..$ : int 0 1 2 3
## ..$ : int 4 5 6 7
## ..$ : int 8 9 10 11
## ..$ : int 12 13 14 15
## ..$ : int 16 17 18 19
## ..$ : int 20 21 22 23
## ..$ : int 24 25 26 27
## ..$ : int 28 29 30 31
## ..$ : int 32 33 34 35
## ..$ : int 36 37 38 39
## ..$ : int 40 41 42 43
## ..$ : int 88 89 90 91
## ..$ : int 92 93 94 95
## ..$ : int 96 97 98 99
## ..$ : int 100 101 102 103
## ..$ : int 104 105 106 107
## ..$ : int 108 109 110 111
## ..$ : int 112 113 114 115
## ..$ : int 116 117 118 119
## ..$ : int 120 121 122 123
## ..$ : int 124 125 126 127
## ..$ : int 128 129 130 131
## ..$ : int 44 45 46 47
## ..$ : int 48 49 50 51
## ..$ : int 52 53 54 55
## ..$ : int 56 57 58 59
## ..$ : int 60 61 62 63
## ..$ : int 64 65 66 67
## ..$ : int 68 69 70 71
## ..$ : int 72 73 74 75
## ..$ : int 76 77 78 79
## ..$ : int 80 81 82 83
## ..$ : int 84 85 86 87
## ..$ : int 264 265 266 267
## ..$ : int 268 269 270 271
## ..$ : int 272 273 274 275
## ..$ : int 276 277 278 279
## ..$ : int 280 281 282 283
## ..$ : int 284 285 286 287 288 289 290 291
## ..$ : int 292 293 294 295
## ..$ : int 296 297 298 299
## ..$ : int 300 301 302 303
## ..$ : int 304 305 306 307
## ..$ : int 308 309 310 311
## ..$ : int 312 313 314 315
## ..$ : int 316 317 318 319
## ..$ : int 320 321 322 323
## ..$ : int 324 325 326 327
## ..$ : int 328 329 330 331 332 333 334 335
## ..$ : int 336 337 338 339
## ..$ : int 340 341 342 343
## ..$ : int 344 345 346 347
## ..$ : int 348 349 350 351
## ..$ : int 176 177 178 179
## ..$ : int 180 181 182 183
## ..$ : int 184 185 186 187
## ..$ : int 188 189 190 191
## ..$ : int 192 193 194 195
## ..$ : int 196 197 198 199
## ..$ : int 200 201 202 203
## ..$ : int 204 205 206 207
## ..$ : int 208 209 210 211
## ..$ : int 212 213 214 215
## ..$ : int 216 217 218 219
## ..$ : int 220 221 222 223
## ..$ : int 224 225 226 227
## ..$ : int 228 229 230 231
## ..$ : int 232 233 234 235
## ..$ : int 236 237 238 239
## ..$ : int 240 241 242 243
## ..$ : int 244 245 246 247
## ..$ : int 248 249 250 251
## ..$ : int 252 253 254 255
## ..$ : int 256 257 258 259
## ..$ : int 260 261 262 263
## ..$ : int 132 133 134 135
## ..$ : int 136 137 138 139
## ..$ : int 140 141 142 143
## ..$ : int 144 145 146 147
## ..$ : int 148 149 150 151
## ..$ : int 152 153 154 155
## ..$ : int 156 157 158 159
## ..$ : int 160 161 162 163
## ..$ : int 164 165 166 167
## ..$ : int 168 169 170 171
## ..$ : int 172 173 174 175
## - attr(*, "group_sizes")= int 4 4 4 4 4 4 4 4 4 4 ...
## - attr(*, "biggest_group_size")= int 8
## - attr(*, "labels")='data.frame': 86 obs. of 4 variables:
## ..$ parent : Factor w/ 3 levels "atrazine","fipronil",..: 1 1 1 1 1 1 1 1 1 1 ...
## ..$ analyte: Factor w/ 8 levels "atrazine","dea",..: 1 1 1 1 1 1 1 1 1 1 ...
## ..$ matrix : Factor w/ 2 levels "amphib","soil": 1 1 1 1 1 2 2 2 2 2 ...
## ..$ time : int 2 4 12 24 48 0 2 4 12 24 ...
## ..- attr(*, "vars")=List of 4
## .. ..$ : symbol parent
## .. ..$ : symbol analyte
## .. ..$ : symbol matrix
## .. ..$ : symbol time
## ..- attr(*, "drop")= logi TRUE## Source: local data frame [352 x 6]
## Groups: parent, analyte, matrix, time [86]
##
## time parent analyte matrix conc replicate
## (int) (fctr) (fctr) (fctr) (dbl) (fctr)
## 1 2 atrazine atrazine amphib 3.068203 1
## 2 2 atrazine atrazine amphib 3.689640 2
## 3 2 atrazine atrazine amphib 4.701166 3
## 4 2 atrazine atrazine amphib 1.892494 4
## 5 4 atrazine atrazine amphib 7.624610 1
## 6 4 atrazine atrazine amphib 4.134300 2
## 7 4 atrazine atrazine amphib 2.050779 3
## 8 4 atrazine atrazine amphib 7.626486 4
## 9 12 atrazine atrazine amphib 2.452278 1
## 10 12 atrazine atrazine amphib 3.786893 2
## .. ... ... ... ... ... ...## Source: local data frame [86 x 7]
## Groups: parent, analyte, matrix [?]
##
## parent analyte matrix time count ConcMean ConcSD
## (fctr) (fctr) (fctr) (int) (int) (dbl) (dbl)
## 1 atrazine atrazine amphib 2 4 3.337876 1.1753225
## 2 atrazine atrazine amphib 4 4 5.359044 2.7518903
## 3 atrazine atrazine amphib 12 4 3.123811 1.2566289
## 4 atrazine atrazine amphib 24 4 1.554919 0.6096102
## 5 atrazine atrazine amphib 48 4 1.001887 0.3545473
## 6 atrazine atrazine soil 0 4 15.930920 2.5911041
## 7 atrazine atrazine soil 2 4 19.029034 4.3534303
## 8 atrazine atrazine soil 4 4 18.770091 2.6953158
## 9 atrazine atrazine soil 12 4 20.614190 3.8918511
## 10 atrazine atrazine soil 24 4 24.996196 4.4473828
## .. ... ... ... ... ... ... ...## <!-- html table generated in R 3.2.3 by xtable 1.8-0 package -->
## <!-- Mon Feb 01 14:35:15 2016 -->
## <table border=1>
## <tr> <th> </th> <th> parent </th> <th> analyte </th> <th> matrix </th> <th> time </th> <th> count </th> <th> ConcMean </th> <th> ConcSD </th> </tr>
## <tr> <td align="right"> 1 </td> <td> atrazine </td> <td> atrazine </td> <td> amphib </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 3.34 </td> <td align="right"> 1.18 </td> </tr>
## <tr> <td align="right"> 2 </td> <td> atrazine </td> <td> atrazine </td> <td> amphib </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 5.36 </td> <td align="right"> 2.75 </td> </tr>
## <tr> <td align="right"> 3 </td> <td> atrazine </td> <td> atrazine </td> <td> amphib </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 3.12 </td> <td align="right"> 1.26 </td> </tr>
## <tr> <td align="right"> 4 </td> <td> atrazine </td> <td> atrazine </td> <td> amphib </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 1.55 </td> <td align="right"> 0.61 </td> </tr>
## <tr> <td align="right"> 5 </td> <td> atrazine </td> <td> atrazine </td> <td> amphib </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 1.00 </td> <td align="right"> 0.35 </td> </tr>
## <tr> <td align="right"> 6 </td> <td> atrazine </td> <td> atrazine </td> <td> soil </td> <td align="right"> 0 </td> <td align="right"> 4 </td> <td align="right"> 15.93 </td> <td align="right"> 2.59 </td> </tr>
## <tr> <td align="right"> 7 </td> <td> atrazine </td> <td> atrazine </td> <td> soil </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 19.03 </td> <td align="right"> 4.35 </td> </tr>
## <tr> <td align="right"> 8 </td> <td> atrazine </td> <td> atrazine </td> <td> soil </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 18.77 </td> <td align="right"> 2.70 </td> </tr>
## <tr> <td align="right"> 9 </td> <td> atrazine </td> <td> atrazine </td> <td> soil </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 20.61 </td> <td align="right"> 3.89 </td> </tr>
## <tr> <td align="right"> 10 </td> <td> atrazine </td> <td> atrazine </td> <td> soil </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 25.00 </td> <td align="right"> 4.45 </td> </tr>
## <tr> <td align="right"> 11 </td> <td> atrazine </td> <td> atrazine </td> <td> soil </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 26.43 </td> <td align="right"> 4.38 </td> </tr>
## <tr> <td align="right"> 12 </td> <td> atrazine </td> <td> dea </td> <td> amphib </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 0.01 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 13 </td> <td> atrazine </td> <td> dea </td> <td> amphib </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.04 </td> <td align="right"> 0.03 </td> </tr>
## <tr> <td align="right"> 14 </td> <td> atrazine </td> <td> dea </td> <td> amphib </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.05 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 15 </td> <td> atrazine </td> <td> dea </td> <td> amphib </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.04 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 16 </td> <td> atrazine </td> <td> dea </td> <td> amphib </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.02 </td> <td align="right"> 0.02 </td> </tr>
## <tr> <td align="right"> 17 </td> <td> atrazine </td> <td> dea </td> <td> soil </td> <td align="right"> 0 </td> <td align="right"> 4 </td> <td align="right"> 0.00 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 18 </td> <td> atrazine </td> <td> dea </td> <td> soil </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 0.00 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 19 </td> <td> atrazine </td> <td> dea </td> <td> soil </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.00 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 20 </td> <td> atrazine </td> <td> dea </td> <td> soil </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.01 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 21 </td> <td> atrazine </td> <td> dea </td> <td> soil </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.01 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 22 </td> <td> atrazine </td> <td> dea </td> <td> soil </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.01 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 23 </td> <td> atrazine </td> <td> dia </td> <td> amphib </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 0.33 </td> <td align="right"> 0.12 </td> </tr>
## <tr> <td align="right"> 24 </td> <td> atrazine </td> <td> dia </td> <td> amphib </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.94 </td> <td align="right"> 0.76 </td> </tr>
## <tr> <td align="right"> 25 </td> <td> atrazine </td> <td> dia </td> <td> amphib </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 1.08 </td> <td align="right"> 0.51 </td> </tr>
## <tr> <td align="right"> 26 </td> <td> atrazine </td> <td> dia </td> <td> amphib </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 1.99 </td> <td align="right"> 1.35 </td> </tr>
## <tr> <td align="right"> 27 </td> <td> atrazine </td> <td> dia </td> <td> amphib </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.66 </td> <td align="right"> 0.53 </td> </tr>
## <tr> <td align="right"> 28 </td> <td> atrazine </td> <td> dia </td> <td> soil </td> <td align="right"> 0 </td> <td align="right"> 4 </td> <td align="right"> 0.00 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 29 </td> <td> atrazine </td> <td> dia </td> <td> soil </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> -0.01 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 30 </td> <td> atrazine </td> <td> dia </td> <td> soil </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.02 </td> <td align="right"> 0.02 </td> </tr>
## <tr> <td align="right"> 31 </td> <td> atrazine </td> <td> dia </td> <td> soil </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.08 </td> <td align="right"> 0.07 </td> </tr>
## <tr> <td align="right"> 32 </td> <td> atrazine </td> <td> dia </td> <td> soil </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.04 </td> <td align="right"> 0.03 </td> </tr>
## <tr> <td align="right"> 33 </td> <td> atrazine </td> <td> dia </td> <td> soil </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.06 </td> <td align="right"> 0.06 </td> </tr>
## <tr> <td align="right"> 34 </td> <td> fipronil </td> <td> fipronil </td> <td> amphib </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 1.87 </td> <td align="right"> 1.35 </td> </tr>
## <tr> <td align="right"> 35 </td> <td> fipronil </td> <td> fipronil </td> <td> amphib </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 1.30 </td> <td align="right"> 0.52 </td> </tr>
## <tr> <td align="right"> 36 </td> <td> fipronil </td> <td> fipronil </td> <td> amphib </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.57 </td> <td align="right"> 0.24 </td> </tr>
## <tr> <td align="right"> 37 </td> <td> fipronil </td> <td> fipronil </td> <td> amphib </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.27 </td> <td align="right"> 0.05 </td> </tr>
## <tr> <td align="right"> 38 </td> <td> fipronil </td> <td> fipronil </td> <td> amphib </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.21 </td> <td align="right"> 0.13 </td> </tr>
## <tr> <td align="right"> 39 </td> <td> fipronil </td> <td> fipronil </td> <td> soil </td> <td align="right"> 2 </td> <td align="right"> 8 </td> <td align="right"> 0.41 </td> <td align="right"> 0.45 </td> </tr>
## <tr> <td align="right"> 40 </td> <td> fipronil </td> <td> fipronil </td> <td> soil </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.87 </td> <td align="right"> 0.24 </td> </tr>
## <tr> <td align="right"> 41 </td> <td> fipronil </td> <td> fipronil </td> <td> soil </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.82 </td> <td align="right"> 0.17 </td> </tr>
## <tr> <td align="right"> 42 </td> <td> fipronil </td> <td> fipronil </td> <td> soil </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.70 </td> <td align="right"> 0.11 </td> </tr>
## <tr> <td align="right"> 43 </td> <td> fipronil </td> <td> fipronil </td> <td> soil </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.36 </td> <td align="right"> 0.06 </td> </tr>
## <tr> <td align="right"> 44 </td> <td> fipronil </td> <td> fipsulf </td> <td> amphib </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 0.35 </td> <td align="right"> 0.29 </td> </tr>
## <tr> <td align="right"> 45 </td> <td> fipronil </td> <td> fipsulf </td> <td> amphib </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.40 </td> <td align="right"> 0.48 </td> </tr>
## <tr> <td align="right"> 46 </td> <td> fipronil </td> <td> fipsulf </td> <td> amphib </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.61 </td> <td align="right"> 0.57 </td> </tr>
## <tr> <td align="right"> 47 </td> <td> fipronil </td> <td> fipsulf </td> <td> amphib </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 1.25 </td> <td align="right"> 0.53 </td> </tr>
## <tr> <td align="right"> 48 </td> <td> fipronil </td> <td> fipsulf </td> <td> amphib </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.91 </td> <td align="right"> 0.57 </td> </tr>
## <tr> <td align="right"> 49 </td> <td> fipronil </td> <td> fipsulf </td> <td> soil </td> <td align="right"> 2 </td> <td align="right"> 8 </td> <td align="right"> 0.01 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 50 </td> <td> fipronil </td> <td> fipsulf </td> <td> soil </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.02 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 51 </td> <td> fipronil </td> <td> fipsulf </td> <td> soil </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.02 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 52 </td> <td> fipronil </td> <td> fipsulf </td> <td> soil </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.02 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 53 </td> <td> fipronil </td> <td> fipsulf </td> <td> soil </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.01 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 54 </td> <td> triadimefon </td> <td> tdla </td> <td> amphib </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 0.06 </td> <td align="right"> 0.02 </td> </tr>
## <tr> <td align="right"> 55 </td> <td> triadimefon </td> <td> tdla </td> <td> amphib </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.08 </td> <td align="right"> 0.05 </td> </tr>
## <tr> <td align="right"> 56 </td> <td> triadimefon </td> <td> tdla </td> <td> amphib </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.04 </td> <td align="right"> 0.02 </td> </tr>
## <tr> <td align="right"> 57 </td> <td> triadimefon </td> <td> tdla </td> <td> amphib </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.02 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 58 </td> <td> triadimefon </td> <td> tdla </td> <td> amphib </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.02 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 59 </td> <td> triadimefon </td> <td> tdla </td> <td> soil </td> <td align="right"> 0 </td> <td align="right"> 4 </td> <td align="right"> 0.00 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 60 </td> <td> triadimefon </td> <td> tdla </td> <td> soil </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 0.00 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 61 </td> <td> triadimefon </td> <td> tdla </td> <td> soil </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.00 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 62 </td> <td> triadimefon </td> <td> tdla </td> <td> soil </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.01 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 63 </td> <td> triadimefon </td> <td> tdla </td> <td> soil </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.01 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 64 </td> <td> triadimefon </td> <td> tdla </td> <td> soil </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.01 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 65 </td> <td> triadimefon </td> <td> tdlb </td> <td> amphib </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 0.08 </td> <td align="right"> 0.02 </td> </tr>
## <tr> <td align="right"> 66 </td> <td> triadimefon </td> <td> tdlb </td> <td> amphib </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.13 </td> <td align="right"> 0.05 </td> </tr>
## <tr> <td align="right"> 67 </td> <td> triadimefon </td> <td> tdlb </td> <td> amphib </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.08 </td> <td align="right"> 0.05 </td> </tr>
## <tr> <td align="right"> 68 </td> <td> triadimefon </td> <td> tdlb </td> <td> amphib </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.04 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 69 </td> <td> triadimefon </td> <td> tdlb </td> <td> amphib </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.03 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 70 </td> <td> triadimefon </td> <td> tdlb </td> <td> soil </td> <td align="right"> 0 </td> <td align="right"> 4 </td> <td align="right"> 0.00 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 71 </td> <td> triadimefon </td> <td> tdlb </td> <td> soil </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 0.01 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 72 </td> <td> triadimefon </td> <td> tdlb </td> <td> soil </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.01 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 73 </td> <td> triadimefon </td> <td> tdlb </td> <td> soil </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.02 </td> <td align="right"> 0.00 </td> </tr>
## <tr> <td align="right"> 74 </td> <td> triadimefon </td> <td> tdlb </td> <td> soil </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.02 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 75 </td> <td> triadimefon </td> <td> tdlb </td> <td> soil </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.04 </td> <td align="right"> 0.01 </td> </tr>
## <tr> <td align="right"> 76 </td> <td> triadimefon </td> <td> triadimefon </td> <td> amphib </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 0.39 </td> <td align="right"> 0.08 </td> </tr>
## <tr> <td align="right"> 77 </td> <td> triadimefon </td> <td> triadimefon </td> <td> amphib </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 0.55 </td> <td align="right"> 0.30 </td> </tr>
## <tr> <td align="right"> 78 </td> <td> triadimefon </td> <td> triadimefon </td> <td> amphib </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 0.75 </td> <td align="right"> 0.32 </td> </tr>
## <tr> <td align="right"> 79 </td> <td> triadimefon </td> <td> triadimefon </td> <td> amphib </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 0.87 </td> <td align="right"> 0.86 </td> </tr>
## <tr> <td align="right"> 80 </td> <td> triadimefon </td> <td> triadimefon </td> <td> amphib </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 0.25 </td> <td align="right"> 0.08 </td> </tr>
## <tr> <td align="right"> 81 </td> <td> triadimefon </td> <td> triadimefon </td> <td> soil </td> <td align="right"> 0 </td> <td align="right"> 4 </td> <td align="right"> 3.11 </td> <td align="right"> 0.85 </td> </tr>
## <tr> <td align="right"> 82 </td> <td> triadimefon </td> <td> triadimefon </td> <td> soil </td> <td align="right"> 2 </td> <td align="right"> 4 </td> <td align="right"> 3.66 </td> <td align="right"> 1.34 </td> </tr>
## <tr> <td align="right"> 83 </td> <td> triadimefon </td> <td> triadimefon </td> <td> soil </td> <td align="right"> 4 </td> <td align="right"> 4 </td> <td align="right"> 5.28 </td> <td align="right"> 0.37 </td> </tr>
## <tr> <td align="right"> 84 </td> <td> triadimefon </td> <td> triadimefon </td> <td> soil </td> <td align="right"> 12 </td> <td align="right"> 4 </td> <td align="right"> 5.93 </td> <td align="right"> 1.00 </td> </tr>
## <tr> <td align="right"> 85 </td> <td> triadimefon </td> <td> triadimefon </td> <td> soil </td> <td align="right"> 24 </td> <td align="right"> 4 </td> <td align="right"> 4.82 </td> <td align="right"> 0.32 </td> </tr>
## <tr> <td align="right"> 86 </td> <td> triadimefon </td> <td> triadimefon </td> <td> soil </td> <td align="right"> 48 </td> <td align="right"> 4 </td> <td align="right"> 4.18 </td> <td align="right"> 0.44 </td> </tr>
## </table>The amphibian data set summary statistics. Atrazine peaks at 4 hours and then declines monotonically. DEA peaks at 12 hours and DIA at 24. Fipronil steadily declines from its first observation at 2 hours with fipronil sulfone not peaking until 24 hours. Triadimenon peaks at 24 hours with its metabolites tdla and tdlb peaking at 4 hours.
## Classes 'grouped_df', 'tbl_df', 'tbl' and 'data.frame': 86 obs. of 7 variables:
## $ parent : Factor w/ 3 levels "atrazine","fipronil",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ analyte : Factor w/ 8 levels "atrazine","dea",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ matrix : Factor w/ 2 levels "amphib","soil": 1 1 1 1 1 2 2 2 2 2 ...
## $ time : int 2 4 12 24 48 0 2 4 12 24 ...
## $ count : int 4 4 4 4 4 4 4 4 4 4 ...
## $ ConcMean: num 3.34 5.36 3.12 1.55 1 ...
## $ ConcSD : num 1.175 2.752 1.257 0.61 0.355 ...
## - attr(*, "vars")=List of 3
## ..$ : symbol parent
## ..$ : symbol analyte
## ..$ : symbol matrix
## - attr(*, "drop")= logi TRUE## Source: local data frame [6 x 7]
## Groups: parent, analyte, matrix [2]
##
## parent analyte matrix time count ConcMean ConcSD
## (fctr) (fctr) (fctr) (int) (int) (dbl) (dbl)
## 1 atrazine atrazine amphib 2 4 3.33787563 1.17532253
## 2 atrazine atrazine amphib 4 4 5.35904387 2.75189034
## 3 atrazine atrazine amphib 12 4 3.12381130 1.25662887
## 4 atrazine atrazine amphib 24 4 1.55491895 0.60961016
## 5 atrazine atrazine amphib 48 4 1.00188749 0.35454734
## 6 atrazine dea amphib 2 4 0.01389877 0.00522407## Source: local data frame [15 x 7]
## Groups: parent, analyte, matrix [3]
##
## parent analyte matrix time count ConcMean ConcSD
## (fctr) (fctr) (fctr) (int) (int) (dbl) (dbl)
## 1 atrazine atrazine amphib 2 4 3.33787563 1.17532253
## 2 atrazine atrazine amphib 4 4 5.35904387 2.75189034
## 3 atrazine atrazine amphib 12 4 3.12381130 1.25662887
## 4 atrazine atrazine amphib 24 4 1.55491895 0.60961016
## 5 atrazine atrazine amphib 48 4 1.00188749 0.35454734
## 6 atrazine dea amphib 2 4 0.01389877 0.00522407
## 7 atrazine dea amphib 4 4 0.04436711 0.02843181
## 8 atrazine dea amphib 12 4 0.05402182 0.01282235
## 9 atrazine dea amphib 24 4 0.03652473 0.01310278
## 10 atrazine dea amphib 48 4 0.01951501 0.01958540
## 11 atrazine dia amphib 2 4 0.32878185 0.12495746
## 12 atrazine dia amphib 4 4 0.94192818 0.76074427
## 13 atrazine dia amphib 12 4 1.08350960 0.50576827
## 14 atrazine dia amphib 24 4 1.98835970 1.34925248
## 15 atrazine dia amphib 48 4 0.66238821 0.53051448## [1] "atrazine"
## [1] "dea"
## [1] "dia"## Source: local data frame [10 x 7]
## Groups: parent, analyte, matrix [2]
##
## parent analyte matrix time count ConcMean ConcSD
## (fctr) (fctr) (fctr) (int) (int) (dbl) (dbl)
## 1 fipronil fipronil amphib 2 4 1.8670398 1.34631230
## 2 fipronil fipronil amphib 4 4 1.3005836 0.52188454
## 3 fipronil fipronil amphib 12 4 0.5659565 0.24026633
## 4 fipronil fipronil amphib 24 4 0.2676732 0.05106111
## 5 fipronil fipronil amphib 48 4 0.2119504 0.13140536
## 6 fipronil fipsulf amphib 2 4 0.3465734 0.28711156
## 7 fipronil fipsulf amphib 4 4 0.3966089 0.47739157
## 8 fipronil fipsulf amphib 12 4 0.6129114 0.57346517
## 9 fipronil fipsulf amphib 24 4 1.2528179 0.52648677
## 10 fipronil fipsulf amphib 48 4 0.9067126 0.56596417## Source: local data frame [0 x 7]
## Groups: parent, analyte, matrix [0]
##
## Variables not shown: parent (fctr), analyte (fctr), matrix (fctr), time
## (int), count (int), ConcMean (dbl), ConcSD (dbl)The soil data set summary statistics. Atrazine in soil showing an upwards trend (need to test if significant) with dia and dea levels very low indicating little degradation in soil over the 48 hour period. Fipronil and triadimenon data also indicating little degradation in soil.
## Source: local data frame [18 x 7]
## Groups: parent, analyte, matrix [3]
##
## parent analyte matrix time count ConcMean ConcSD
## (fctr) (fctr) (fctr) (int) (int) (dbl) (dbl)
## 1 atrazine atrazine soil 0 4 15.930919692 2.591104068
## 2 atrazine atrazine soil 2 4 19.029034412 4.353430337
## 3 atrazine atrazine soil 4 4 18.770091080 2.695315758
## 4 atrazine atrazine soil 12 4 20.614190363 3.891851074
## 5 atrazine atrazine soil 24 4 24.996195532 4.447382801
## 6 atrazine atrazine soil 48 4 26.434744900 4.377099498
## 7 atrazine dea soil 0 4 0.000000000 0.000000000
## 8 atrazine dea soil 2 4 0.001570429 0.000633009
## 9 atrazine dea soil 4 4 0.002999216 0.001537652
## 10 atrazine dea soil 12 4 0.005469015 0.004234880
## 11 atrazine dea soil 24 4 0.007308439 0.001736211
## 12 atrazine dea soil 48 4 0.008784632 0.003772339
## 13 atrazine dia soil 0 4 0.000000000 0.000000000
## 14 atrazine dia soil 2 4 -0.007727286 0.007374085
## 15 atrazine dia soil 4 4 0.020538600 0.020166308
## 16 atrazine dia soil 12 4 0.077036921 0.067772652
## 17 atrazine dia soil 24 4 0.042975804 0.030007793
## 18 atrazine dia soil 48 4 0.063693292 0.058241606## Source: local data frame [10 x 7]
## Groups: parent, analyte, matrix [2]
##
## parent analyte matrix time count ConcMean ConcSD
## (fctr) (fctr) (fctr) (int) (int) (dbl) (dbl)
## 1 fipronil fipronil soil 2 8 0.40768123 0.447265792
## 2 fipronil fipronil soil 4 4 0.86826316 0.244191727
## 3 fipronil fipronil soil 12 4 0.81738069 0.169691335
## 4 fipronil fipronil soil 24 4 0.70225877 0.108745058
## 5 fipronil fipronil soil 48 4 0.35560948 0.058543752
## 6 fipronil fipsulf soil 2 8 0.01022479 0.012898421
## 7 fipronil fipsulf soil 4 4 0.02193831 0.010765665
## 8 fipronil fipsulf soil 12 4 0.01829893 0.005013814
## 9 fipronil fipsulf soil 24 4 0.01917848 0.009139211
## 10 fipronil fipsulf soil 48 4 0.01262887 0.005517408## Source: local data frame [0 x 7]
## Groups: parent, analyte, matrix [0]
##
## Variables not shown: parent (fctr), analyte (fctr), matrix (fctr), time
## (int), count (int), ConcMean (dbl), ConcSD (dbl)pdf(paste(micro.graphics,"data_mean_scatterplot",".pdf", sep=""))
par(mfrow=c(3,1))
print(parents)## [1] atrazine triadimefon fipronil
## Levels: atrazine fipronil triadimefon for(parent in parents){
i=0
print(parent)
temp.parent <- micro.group.stats.amphib[which(micro.group.stats.amphib$parent==parent),]
print(temp.parent)
parent.analytes <- unique(temp.parent$analyte)
for(analyte in parent.analytes){
print(parent)
print(analyte)
analytetemp <- micro.group.stats.amphib[which(micro.group.stats.amphib$analyte==analyte),]
xvalues <- as.numeric(as.character(analytetemp$time))
points.y <- micro.amphib[which(micro.amphib$analyte==analyte),]$conc
points.x <- as.numeric(as.character(micro.amphib[which(micro.amphib$analyte==analyte),]$time))
#create empty plot if needed
if(i==0){
parenttemp <- micro.group.stats.amphib[which(micro.group.stats.amphib$parent==parent),]
maxconc <- max(points.y)
plot(xvalues,analytetemp$ConcMean,type="l", xlim=c(0,48),
ylim = c(0,maxconc), main=parent,col="black", xlab="Hours", ylab="Concentration")
axis(1,at=xvalues)
i=1
}
#add line for parent metabolite
if(analyte %in% parents){
lines(xvalues,analytetemp$ConcMean,type="l",col="red")
points(points.x, points.y, col = "red")
}else{
#add line for daughter metabolite
lines(xvalues,analytetemp$ConcMean,type="l",col="blue")
points(points.x, points.y, col = "blue")
}
}
}## [1] "atrazine"
## Source: local data frame [15 x 7]
## Groups: parent, analyte, matrix [3]
##
## parent analyte matrix time count ConcMean ConcSD
## (fctr) (fctr) (fctr) (int) (int) (dbl) (dbl)
## 1 atrazine atrazine amphib 2 4 3.33787563 1.17532253
## 2 atrazine atrazine amphib 4 4 5.35904387 2.75189034
## 3 atrazine atrazine amphib 12 4 3.12381130 1.25662887
## 4 atrazine atrazine amphib 24 4 1.55491895 0.60961016
## 5 atrazine atrazine amphib 48 4 1.00188749 0.35454734
## 6 atrazine dea amphib 2 4 0.01389877 0.00522407
## 7 atrazine dea amphib 4 4 0.04436711 0.02843181
## 8 atrazine dea amphib 12 4 0.05402182 0.01282235
## 9 atrazine dea amphib 24 4 0.03652473 0.01310278
## 10 atrazine dea amphib 48 4 0.01951501 0.01958540
## 11 atrazine dia amphib 2 4 0.32878185 0.12495746
## 12 atrazine dia amphib 4 4 0.94192818 0.76074427
## 13 atrazine dia amphib 12 4 1.08350960 0.50576827
## 14 atrazine dia amphib 24 4 1.98835970 1.34925248
## 15 atrazine dia amphib 48 4 0.66238821 0.53051448
## [1] "atrazine"
## [1] "atrazine"## [1] "atrazine"
## [1] "dea"
## [1] "atrazine"
## [1] "dia"
## [1] "triadimefon"
## Source: local data frame [15 x 7]
## Groups: parent, analyte, matrix [3]
##
## parent analyte matrix time count ConcMean ConcSD
## (fctr) (fctr) (fctr) (int) (int) (dbl) (dbl)
## 1 triadimefon tdla amphib 2 4 0.05514470 0.021089764
## 2 triadimefon tdla amphib 4 4 0.08150175 0.046221502
## 3 triadimefon tdla amphib 12 4 0.03632384 0.015222899
## 4 triadimefon tdla amphib 24 4 0.02400790 0.007522554
## 5 triadimefon tdla amphib 48 4 0.01549908 0.004845715
## 6 triadimefon tdlb amphib 2 4 0.08362714 0.016364595
## 7 triadimefon tdlb amphib 4 4 0.13009543 0.054315693
## 8 triadimefon tdlb amphib 12 4 0.07664673 0.048553355
## 9 triadimefon tdlb amphib 24 4 0.04183235 0.011652266
## 10 triadimefon tdlb amphib 48 4 0.02950214 0.003438978
## 11 triadimefon triadimefon amphib 2 4 0.38678831 0.075852995
## 12 triadimefon triadimefon amphib 4 4 0.54862458 0.300394386
## 13 triadimefon triadimefon amphib 12 4 0.75491439 0.318237965
## 14 triadimefon triadimefon amphib 24 4 0.86540039 0.860421208
## 15 triadimefon triadimefon amphib 48 4 0.24868748 0.079848623
## [1] "triadimefon"
## [1] "tdla"## [1] "triadimefon"
## [1] "tdlb"
## [1] "triadimefon"
## [1] "triadimefon"
## [1] "fipronil"
## Source: local data frame [10 x 7]
## Groups: parent, analyte, matrix [2]
##
## parent analyte matrix time count ConcMean ConcSD
## (fctr) (fctr) (fctr) (int) (int) (dbl) (dbl)
## 1 fipronil fipronil amphib 2 4 1.8670398 1.34631230
## 2 fipronil fipronil amphib 4 4 1.3005836 0.52188454
## 3 fipronil fipronil amphib 12 4 0.5659565 0.24026633
## 4 fipronil fipronil amphib 24 4 0.2676732 0.05106111
## 5 fipronil fipronil amphib 48 4 0.2119504 0.13140536
## 6 fipronil fipsulf amphib 2 4 0.3465734 0.28711156
## 7 fipronil fipsulf amphib 4 4 0.3966089 0.47739157
## 8 fipronil fipsulf amphib 12 4 0.6129114 0.57346517
## 9 fipronil fipsulf amphib 24 4 1.2528179 0.52648677
## 10 fipronil fipsulf amphib 48 4 0.9067126 0.56596417
## [1] "fipronil"
## [1] "fipronil"## [1] "fipronil"
## [1] "fipsulf"dev.off()## png
## 2la de da
turn times series to zoo objects
estimate lambdas using maximum likelihood for each time series
library(maxLik)
MLexp <- function(times, data){
expLik <- function(param) { y <- data t <- times alpha1 <- param[1] lambda1 <- param[2]-1(alpha1-lambda1t) - y/(exp(alpha1-lambda1*t)) }
max.fit <- maxLik(expLik, start = c(1,1)) crit <- qt(.975, length(data)-2) max.intercept.CI <- c(summary(max.fit)\(est[1,1]-crit*summary(max.fit)\)est[1,2],summary(max.fit)\(est[1,1]+crit*summary(max.fit)\)est[1,2]) max.decayrate.CI <- c(-1summary(max.fit)\(est[2,1]-crit*summary(max.fit)\)est[2,2],-1summary(max.fit)\(est[2,1]+crit*summary(max.fit)\)est[2,2]) max.halflife.CI <- c(log(2)/max.decayrate.CI[2],log(2)/max.decayrate.CI[1])
paste(“MLE for Initial Concentration =”,max.fit\(est[1], "MLE for decay rate =", max.fit\)est[2], “MLE for half life =”, -log(.5)/max.fit$est[2])
temp.list <- list(max.fit\(est[1], max.fit\)est[2], -log(.5)/max.fit$est[2], max.intercept.CI, max.decayrate.CI, max.halflife.CI, vcov(max.fit) ) return(temp.list) }
MLexp.intercept <- function(times, data) { temp.fit <- MLexp(times,data) temp.fit[[1]] }
MLexp.decayrate <- function(times, data) { temp.fit <- MLexp(times,data) temp.fit[[2]] }
MLexp.halflife <- function(times,data) { temp.fit <- MLexp(times,data) temp.fit[[3]] }
MLexp.intercept.CI <- function(times,data){ temp.fit <- MLexp(times,data) temp2 <- temp.fit[[4]] names(temp2) <- c(“Lower95”, “Upper95”) temp2 }
MLexp.decayrate.CI <- function(times,data){ temp.fit <- MLexp(times,data) temp2 <- temp.fit[[5]] names(temp2) <- c(“Lower95”, “Upper95”) temp2 }
MLexp.halflife.CI <- function(times,data){ temp.fit <- MLexp(times,data) temp2 <- temp.fit[[6]] names(temp2) <- c(“Lower95”, “Upper95”) temp2 }
microsome experiment analysis
data in csv format
